中国临床药学杂志2012，Vol.21Issue(2)：109-112,4.
超高效液相色谱法测定人血清中多索茶碱浓度
Determination of doxofylline concentration in human serum by ultra high performance liquid chromatography
汪永忠 1李颖 1高家荣 1夏伦祝1
作者信息
- 1. 安徽中医学院第一附属医院药学部,合肥230031
- 折叠
摘要
Abstract
AIM To establish a method for determining the concentration of doxofylline in human serum by ultra high performance liquid chromatography (UPLC). METHODS The column was Waters Acquity C18(50 mm× 2.1 mm, 1.7 μm), the mobile phase was acetonitrile-water in gradient programs at flow rate of 0.2 mL· min-1, and the caffeinewas used as internal standard. The temperature of column was set at room temperature and the detection wavelength was set at 273 nm. RESULTS The calibration curves were linear in the range of 0.18-23.20 mg·L-1 for doxofylline;the linear regression equation was y= 0.181 8x + 0.005 l(r = 0.999 9, n = 10);the RSD of intra-day and inter-day was below 15 %; the recovery rates were 94.70% - 106.16%. CONCLUSION The method is sensitive, rapid and accurate, which is usable for monitoring of doxofylline and pharmacokinetic studies.关键词
多索茶碱/超高效液相色谱法/血药浓度监测Key words
doxofylline/ ultra high performance liquid chromatography/ serum concentration monitoring引用本文复制引用
汪永忠,李颖,高家荣,夏伦祝..超高效液相色谱法测定人血清中多索茶碱浓度[J].中国临床药学杂志,2012,21(2):109-112,4.
