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东风菜叶柄无性系建立研究

乔雪 姜宇 王巍 张冬艳 姜长阳

中国林副特产Issue(2):4-6,3.
中国林副特产Issue(2):4-6,3.

东风菜叶柄无性系建立研究

The Study on Establishment of the Clone of Doellingeria scaber

乔雪 1姜宇 1王巍 1张冬艳 1姜长阳1

作者信息

  • 1. 辽宁师范大学生命科学学院,辽宁大连116081
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摘要

Abstract

The aim was to preserve the wild resources and meet the needs of false planting,stipes of Doellingeria scaber were used as material to do the research on callus inducement and differentiation,rooting of adventitious buds and rooting multiplication of tube seedlings,transplantation of tube seedlings and established the clone of variation plants.The results indicate that the best medium for callus induction of stipes was MS+2,4-D2.5mg/L;MS+GA30.5mg/L+AgNO31.5mg/L+NAA 0.1mg/L was suitable for callus differentiation;the ideal method for rooting of adventitious buds and tube seedlings was cultivated in the medium White+IAA 0.1mg/L after the incisions were dealed with aseptic solution of NAA 20mg/L;the test tube seedlings transplanted maintained all botany characteristics of the wild Doellingeria scaber.

关键词

东风菜/愈伤组织/快速繁殖

Key words

Doellingeria scaber/Callus/Rapid Propagation

分类

农业科技

引用本文复制引用

乔雪,姜宇,王巍,张冬艳,姜长阳..东风菜叶柄无性系建立研究[J].中国林副特产,2012,(2):4-6,3.

基金项目

辽宁省高等教育教学改革资助项目 ()

辽宁师范大学教学改革资助项目 ()

中国林副特产

1001-6902

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