中国免疫学杂志2012，Vol.28Issue(3)：205-209,216,6.DOI:10.3969/j.issn.1000-484X.2012.03.004
CD147抗体对破骨细胞中基质金属蛋白酶活性影响的体外研究
Effect of CD147 monoclonal antibody on activity of matrix metalloproteinase on differentiation of osteoclast in vitro
余世明 1初同伟 1廖通权 1胡旭 1刘栓得 1周跃1
作者信息
- 1. 第三军医大学新桥医院骨科,重庆400037
- 折叠
摘要
Abstract
Objective:To establish the model of differentiation and maturation of osteoclast (Ocs) in vitro and to studies the effect of CD147 monoclonal antibody on matrix metalloproteinases-2,9 expressed in differentiation of osteoclast in vitro. Methods:Ocs were induced by peripheral blood mononuclear cells(PBMCs)of human using medium with macrophage colony stimulating factor ( M-CSF ) and receptor activator of nuclear factor-kappaB ligand ( RANKL). PBMCs were divided into 2 groups, antibody group ( RANKL + M-CSF + CD147Ab) and control group (RANKL + M-CSF). The maturation of osteoclasts was observed by tartrate-resistant acid phos-phatase (TRAP) staining and the function of osteoclasts was analyzed by bone resorption test The mRNA levels of CD147 ,MMP-2 and MMP-9 were detected by Real-Time PCR. And the activity of MMP-2, MMP-9 expressed by osteoclast was assessed by zymography analyses. Results :①The formation of osteoclast-like cells and bone resorption can be detected by TRAP staining and bone resorption test in control group, while the formation and activity of Ocs were inhibited in antibody group. ②The mRNA levels of CD147, MMP-2 and MMP-9 of the antibody group at 24 h,48 h time points were lower than the corresponding control group(P <0. 05). Moreover,the mRNA levels of MMP-2,MMP-9 were significantly positively correlated to the mRNA levels of CD147. ③The digestion capacity of MMP-2, MMP-9 zymogen and active enzyme of the antibody group at 24 h,48 h time points were lower than the corresponding control group's (P <0. 05) by Gelatin zymography analyses. Conclusion :CD147 monoclonal antibody can inhibit the expression and activity of MMP-2 and MMP-9 in the differentiation and maturation of osteoclast. One of the possible mechanisms activation of Ocs regulated by CD147 may be achieved by regulating the expression and activity of MMP-2 and MMP-9.关键词
破骨细胞/CD147/基质金属蛋白酶/明胶酶谱Key words
Osteoclasts/CD147/Matrix metalloproteinases/Gelatin zymography analyses分类
医药卫生引用本文复制引用
余世明,初同伟,廖通权,胡旭,刘栓得,周跃..CD147抗体对破骨细胞中基质金属蛋白酶活性影响的体外研究[J].中国免疫学杂志,2012,28(3):205-209,216,6.
