中国免疫学杂志2012，Vol.28Issue(3)：210-212,216,4.DOI:10.3969/j.issn.1000-484X.2012.03.005
人肺癌PG细胞培养上清液对PBMC凋亡的影响
Effects of PG cell culture supernatant on apoptosis of peripheral blood mononuclear cell
摘要
Abstract
Objective; To analyze the influence of PG cells culture supernatant on proliferation inhibition and apoptosis of PB-MC and investigate the probable mechanism. Methods: Different supernatants from PG cells were added into the culture system of PB-MC. The PBMC were obtained after 24,72 h of treatment, and the PBMC cell cycle, apoptosis rate were detected using flow cytometry. In addition, the change of apoptosis related proteins Bcl-2, Fas were detected by flow cytometry and Western blot, respectively. Results: The addition of the supernatant from PG cell culture system did not inhibit PBMC into cell cycle but the apoptosis rate of PBMC showed significantly increased. At the same time,we found that supernatant of PG cell can regulate the expression of apoptosis related proteins Bcl-2 and Fas. Conclusion: PG secretions were able to reduce the number of PBMC may be related to the supernatant of PG cell can regulate the expression of apoptosis related proteins and induce the PBMC apoptosis.关键词
PG细胞培养上清/PBMC/免疫逃逸Key words
PG cell culture supernatant/PBMC/Immune escape分类
医药卫生引用本文复制引用
张铸业,王彦刈,高国丽,卢忠英..人肺癌PG细胞培养上清液对PBMC凋亡的影响[J].中国免疫学杂志,2012,28(3):210-212,216,4.基金项目
本文受国家自然科学基金项目(30960443)、贵州省国际科技合作计划项目([2009 ]700103)和人事部留学人员科技活动项目择优资助经费资助([ 2009 ]4) （30960443）
