中国免疫学杂志2012，Vol.28Issue(3)：221-225,230,6.DOI:10.3969/j.issn.1000-484X.2012.03.008
PD-L1在人胎盘源间充质干细胞对脐血CD8+T细胞免疫调节中的作用
The effect of PD-L1 in immunoregulation of human placenta mesenchymal stem cells on cord blood CD8 +T cells
摘要
Abstract
Objective: To investigate the effect of PD-L1 in human placenta derived mesenchymal stem cells (hPMSCs) mediating immunoregulation on cord blood CD8+ T cell activation, cell cycle and secretion of IL-17. Methods: The expression of the PD-L1 on hPMSCs was detected by RT-PCR and FCM respectively. Specific PD-L1 siRNAs were transfected into hPMSCs via cathodolyte liposome transfection method. CD8+ T cells were sorted from cord blood with immunomagetic beads. The expression of early activation pheno-type, cell cycle and cytokine secretion of cord blood CD8 + T cells were analyzed by FCM. Results: PD-L1 siRNA could effectively block the expression of PD-L1 which was highly expressed on hPMSCs. The expression of CD69 on cord blood CD8 + T cells had no significantly difference between the blocking groups and the unblocking groups. Compared with the unblocking groups, the number of cord blood CD8 + T cells in G0/G1 phase was decreased while the number of cord blood CD8 + T cells in S phase was increased in the blocking groups. hPMSCs caused a sharp increase of IL-17 secretion which was further up-regulated in blocking group. Conclusion: PD-L1 expressed on hPMSCs could promote the inhibitory effect of hPMSCs on cord blood CD8 + T cell cycle, and inhibit the up-regulation of hPMSCs mediated on the expression level of IL-17 secreted by cord blood CD8 + T cells.关键词
hPMSCs/CD8+T/PD-L1/细胞周期/IL-17Key words
hPMSCs/ CD8+ T cells/ PD-L1/ Cell cycle/ IL-17分类
医药卫生引用本文复制引用
王国艳,李广云,王斐斐,张思英,都海波,栾希英..PD-L1在人胎盘源间充质干细胞对脐血CD8+T细胞免疫调节中的作用[J].中国免疫学杂志,2012,28(3):221-225,230,6.基金项目
本文受山东省科技发展计划资助(2011GGH21818)、山东省自然科学基金资助(Y2006C2)、山东省医药卫生发展计划资助(2007 HZ039)和滨州医学院科研启动基金资助(BY2007KYQD09) （2011GGH21818）
