中国免疫学杂志2012，Vol.28Issue(3)：242-245,4.DOI:10.3969/j.issn.1000-484X.2012.03.013
广州无偿献血人群中丙型肝炎抗体阳性者HCV基因型与病毒载量的关系
The correlation of HCV genotypes and viral loads among volunteer blood donors in Guangzhou
摘要
Abstract
Objective:To determine the lastest correlation between HCV genotypes and viral loads among volunteer blood donors in Guangzhou. Methods;605 anti-HCV positive plasma samples were obtained from volunteer blood donors in Guangzhou during year of 2008 to 2011. Firstly,HCV RNA in those samples were detected by quantitative polymerase chain reaction (Q-PCR). Futher, NS5B gene were detected by Q-PCR followed by direct DNA sequencing and phylogenetic analysis in HCV RNA positive samples. Results ;337 samples were found HCV RNA positive in 605 anti-HCV positive plasma samples. 320 samples (95% ,320/337) were genotyped (based on NS5B gene) in 337 HCV RNA positive samples,and subtypes HCV lb,6a,3a,2a,3b,la,6n were detected at frequencies of 45. 00% ,33. 44% ,8. 75% ,7. 81% ,4. 38% ,0. 31% ,0. 31% Respectively. HCVlb was associated with a significantly (P<0.05) higher viral load compared to HCV6a,2a and 3a,while HCV6a also had a relatively higher viral load than HCV 2a and 3 a Conclusion; HCV lb and 6a were the most predominant subtypes due to the higher viral load than the other subtypes among volunteer blood donors in Guangzhou.关键词
丙肝病毒(HCV)/基因测序/基因分型/病毒载量/无偿献血者Key words
Hepatitis C virus (HCV) /Gene sequencing/HCV genotyping/Viral loads/Volunteer blood donors分类
医药卫生引用本文复制引用
廖峭,王继红,许茹,王敏,黄珂,熊华平,叶欣,戎霞,罗广平,付涌水..广州无偿献血人群中丙型肝炎抗体阳性者HCV基因型与病毒载量的关系[J].中国免疫学杂志,2012,28(3):242-245,4.基金项目
本文为国家自然科学基金(No.30872162)、广州市医药卫生重大项目(No.2009-ZDa-05)、2010年广州市科技局重大科技专项(No.2010U1E00661) （No.30872162）
