中国免疫学杂志2012，Vol.28Issue(5)：385-388,4.DOI:10.3969/j.issn.1000-484X.2012.05.001
ABCG2单抗联合NPs-PTX体外抑制MM CSCs作用初探
Preliminary study of inhibiting MM CSCs by ABCG2 monoclonal antibody in combination with NPs-PTX in vitro
摘要
Abstract
Objective:To deled the inhibilive effecl on multiple myeloma (MM) cancer stem cells(CSCs) by ABCG2 monoclonal anlibody (McAb) in combination wilh Fe3O4 nanoparticles-paclitaxel (NPs-PTX) in vitro. Methods; MM CSCs (CD138~ CD34 ~ ) were sorled from human MM cell lines NCI-H929 and RPMI8226 through the immune magnetic beads sorling method. Growlh inhibitive effect of ABCG2 McAb in combination with NPs-PTX on MM CSCs was delecled by CCK-8 assay. The migration rale of MM CSCs was delecled by transwell assay. Effecls of cell cycles and apoplosis were also delecled by flow cylomelry (FCM). Results;The proliferation of NCI-H929 and RPMI8226 cells were suppressed after trealmenl wilh ABCG2 McAb in combination wilh NPs-PTX for 24 hours. The growlh inhibilion rale was 70% in lhe NCI-H929 cells or 80% in lhe RPMI8226 cells. There was slalislical significant difference (P<0.05) between lhe combined utilization of ABCG2 McAb and NPs-PTX group (80% or 70% ) in contrast to lhe utilization of NPs-PTX group alone (55% ). As to lhe rate of migralion, ABCG2 McAb in combination wilh NPs-PTX group was 0. 6% but that of NPs-PTX group was 1.7%. The difference was statistically significant (P < 0. 01). ABCG2 McAb in combination with NPs-PTX could effectively induce MM CSCs apoplosis, and early apoplosis rate reached 43. 15%. Compared to NPs-PTX group (18. 35% ), there was slalislical significant difference (P <0. 05) in the combined group. In addilion, ABCG2 McAb togelher with NPs-PTX could make MM CSCs cell cycle blocked in G/M period. Conclusion: ABCG2 McAb in combination with NPs-PTX has lhe function of inhibiting MM CSCs in vitro, which may associate with induction of cell apoptosis and interruption of cell cycle.关键词
多发性骨髓瘤/癌干细胞/Fe3O4纳米紫杉醇/ABCG2/体外抑制Key words
Multiple myeloma/ Cancer slem cells/ Fe3O4 nanoparlicles-paclilaxel/ ABCG2/ Inhibilion in vitro分类
医药卫生引用本文复制引用
杨翠萍,熊非,窦骏,刘云婧,陈峻崧,顾宁..ABCG2单抗联合NPs-PTX体外抑制MM CSCs作用初探[J].中国免疫学杂志,2012,28(5):385-388,4.基金项目
本文受973项目 (No.2011CB933500)和江苏省研究生科研创新计划项目(3060632501)资助 （No.2011CB933500）
