中国免疫学杂志2012，Vol.28Issue(5)：389-392,4.DOI:10.3969/j.issn.1000-484X.2012.05.002
不同毒力结核分枝杆菌感染小鼠对肺泡巨噬细胞的凋亡率及其时相性变化的影响
Detection of apoptosis rate of alveolar macrophages in mice and change with the time for infection of various kinds of poisonous mycobacterium tuberculosis
摘要
Abstract
Objective:To analyze and study alveolar macrophages of mice' apoptosis rale and Lime phase change infecled by various of poisonous mycobaclerium tuberculosis. Methods: The mice were injecled with mycobacterium tuberculosis bacteria suspension through the Lait vein of mice Lo replicale models of mice infecled wilh Mycobaclerium tuberculosis. After the successful replication of the models, we cleaved the alveolar of every group of mice and collect cleavage liquid lo oblain alveolar macrophages of the infecled mice on the lsl,3rd,5th,7th,9th,11th,13th,15th days, then laser co focal microscopy was used lo observe the model of alveolar macrophages of mice infecled by mycobaclerium tuberculosis. At the same time, macrophage' apoplosis rale was delecled by flow cylomelry and apoplosis rale of changing alveolar macrophages of every group was compared and analysed in differenl limes. Results:The resulls of laser co focal microscopy showed that Mycobaclerium tuberculosis H37Rv and BCG were swallowed largely by alveolar macrophage of mice. The resulls of flow cylomelry showed that apoplosis rale of alveolar macrophages of the mice infecled by Mycobaclerium tubercu-losis H37Rv and BCG gradually increased on the first 9 days and reached the maximum in the ninth day, wilh the lime goes on, then gradually decreased. The apoplosis rale of alveolar macrophages of the mice infecled by Mycobaclerium tuberculosis H37Rv was higher than the BCG groups,the two groups was slalislically significant(P <0. 05 ). Conclusion:The apoplosis rale of alveolar macrophages of the mice is relaled lo the virulence of Mycobaclerium tuberculosis.关键词
结核分枝杆菌/肺泡巨噬细胞/凋亡Key words
Mycobaclerium tuberculosis/ Alveolar macrophage/ Apoplosis分类
医药卫生引用本文复制引用
董江涛,徐芳,田玺择,姚楠,吴芳,章乐,王远志,曹旭东,张万江..不同毒力结核分枝杆菌感染小鼠对肺泡巨噬细胞的凋亡率及其时相性变化的影响[J].中国免疫学杂志,2012,28(5):389-392,4.基金项目
本文为国家自然科学基金资助项目(No.81160192,30960355,30760237)、石河子大学科学技术研究发展计划"自然科学与计划创新"重点项目(No.ZRKX2010ZD01)、石河子大学2010年研究生创新基金项目(No.YJCX2010-Y07) （No.81160192,30960355,30760237）
