中国免疫学杂志2012，Vol.28Issue(5)：428-432,5.DOI:10.3969/j.issn.1000-484X.2012.05.011
人AAT基因在糖尿病细胞治疗中对β细胞的免疫保护作用
The immunosuppressive and protective ability of hAAT for beta cells transplantation in diabetes
摘要
Abstract
Objective:To investigate the protective effect of human α 1-antitrypsin (hAAT) in the cell transplantation of diabetes. We constructed NIT-hAAT cells, which can express human α1-antitrypsin (hAAT) gene stably. Methods:Transfect NIT-1 cells with the eukaryotic expression vector of hAAT. The positive clones were screened and called NIT-hAAT cells. These cells were transplanted into the subrenal capsule of diabetes model mice, and then detect the blood glucose, serum insulin level and survival time of mice from different transplantation groups, so that to observe the effect of distinct treatment in diabetes. Besides, in order to observed the immune protection of hAAT in vivo, the expression of hAAT in transplant parts were detected by RT-RCP and the biopsy of tissues were stained by hematoxylin and eosin( HE). Results: After NIT-hAAT cells transplantation, the blood glucose levels of mice declined effectively and maintained to be normal. The expression of hAAT can prolong the survival time of graft. Transplantation with NIT-hAAT cells could enhance the survival rate of diabetes mice (P <0. 05) . Besides, the result of pathology test with HE staining confirmed that the hAAT could significantly reduce graft inflammation. But the expression of hAAT was gradually decreased in vivo by the RT-PCR, which was indicated that the Nit-hAAT cells were loss their function eventually by immune rejection. Conclusion; NIT-hAAT cells expressing hAAT can inhibit the immune rejection and prolong the survival time of β cells.关键词
α1-抗胰蛋白酶/糖尿病/细胞移植/移植排斥/免疫调节Key words
Alpha 1-antitrypsin/ Diabetes/ Cells transplantation/ Transplant rejection/ Immune regulation分类
医药卫生引用本文复制引用
廖玉婷,叶剑,李青,张晓丹,齐晖,李富荣..人AAT基因在糖尿病细胞治疗中对β细胞的免疫保护作用[J].中国免疫学杂志,2012,28(5):428-432,5.基金项目
本文为国家973前期专项(2007CB516811)和深圳市科技计划项目(201001005) （2007CB516811）
