中国免疫学杂志2012，Vol.28Issue(5)：449-452,4.DOI:10.3969/j.issn.1000-484X.2012.05.016
IL-1β和IL-18在鼠巨细胞病毒播散性感染中的表达及意义
The expressions of IL-1β and IL-18 in mice with disseminated murine cytomegalovirus infection
摘要
Abstract
Objective: To investigate the expressions of IL-1β and IL-18 in mice with disseminated murine cytomegalovirus (MCMV) infection, this study characterized the expression of caspase-1, and the distribution of IL-1β and IL-18 in different organs. Methods:BALB/c mice were randomly divided into two groups. One was infected with MCMV Smith for establishing disseminated infection; the other was sham-inoculated control. On days 0 (representing uninfected control mice) , 3, 7, 14 of the experiment, four mice of each group were randomly chosen to be killed separately. The viral titers in tissues of salivary gland, lung and liver were quantified by a standard plaque assay. The expressions of procaspase-1 and caspase-1 were detected by Western blot. The levels of IL-1β and IL-18 in sera were measured by double antibody sandwich ELISA, and their expressions in different organs were detected by im-munohistochemistry. Results: MCMV titers in livers quickly increased to peak levels on day 3 after infection but quickly declined. Titers in the lung were undetectable throughout the two weeks. In the salivary gland, viral titers gradually increased after infection. The expressions of procaspase-1 and caspase-1 in spleen were significantly increased and peaked on day 3 but gradually decreased afterwards. Meanwhile, the levels of IL-1β and IL-18 in sera of MCMV-infected mice were significantly higher than those in controls (all P < 0.01). Conclusion: These results demonstrate that the inflammasome has been actived and converted procaspase-1 to caspase-1, and the levels of IL-1β and IL-18 in sera are significantly increased and the cytokines display organ-specific distribution after MCMV infection.关键词
鼠巨细胞病毒/caspase-1/IL-1β/IL-18Key words
Murine cytomegalovirus (MCMV) /caspase-l /Interleukin-1β/Interleukin-18分类
医药卫生引用本文复制引用
李旭芳,刘玲玲,刘兴楼,舒赛男,李革,方峰..IL-1β和IL-18在鼠巨细胞病毒播散性感染中的表达及意义[J].中国免疫学杂志,2012,28(5):449-452,4.基金项目
本文为教育部博士点基金资助项目(20090142110076) （20090142110076）
