中国免疫学杂志2012，Vol.28Issue(5)：458-461,464,5.DOI:10.3969/j.issn.1000-484X.2012.05.018
骨髓造血细胞岛在免疫相关性血细胞减少患者中的异质性研究
The heterogeneity of hematopoietic islands in immuno-related hematocytopenia
摘要
Abstract
Objective:To study the different characteristics of hematopoietic islands(HI) in immuno-related hematocytopenia (IRH)/ immuno-related pancytopenia( IRP) , study the relationship between it and MHC molecules and antihuman globulin of myeloid element (IgG) , investigate the relevant immune mechanisms and the guiding significance for clinical diagnosis and treatment. Methods ; ①Wright's Staining was utilized for observing the immunocyte quantity and hematopoietic cell component in myeloid element islands of 37 patients with IRH/IRP;②Cytochemical staining was utilized for observing the peroxidase (POX) activity and the condition of iron storage of immune cells in HI;③Immunochemistry staining was utilized for observing the MHC II (HLA-DR antigen) of antigen presenting cell in the bone marrow;④Immunofiuorescence (IF) staining was utilized for observing anti-human IgG antibody in hematopoietic cells and immune cells;⑤Observing different types of HI in the bone marrow. Results:①The hematopoietic cells and immune cells were various in HI,and were closely related with ESR, IgG, serum HLA-B27, peroxidase ( POX) , HLA-DR and IF. ②According to the biological properties of immune cells,the HI were divided into five types;antibody dependent cell cytotoxicity ( ADCC) type, macrophages (Mψ) type, antigen presenting type, plasma cells type and promyelocytic type. ③Antihuman IgG fluorescent antibodies were expressed in all naive hematopoietic cell membranes of HI,and different strong positive IF particles were present in the contact position between immune cells and hematopoietic cells and the cytoplasms of antigen presenting cells ( APC) ;④Mψ-type HI was the most in number, and was often coexisted with ADCC-type HI in patients with higher erythrocyte sedimentation rate(ESR). In the bone marrow of patients with both HLA-B27 and ESR increase, ADCC-type HI, Mψ-type HI and AP-type HI could been seen at the same time. Conclusions: The basis of HI is that the change of immature hematopoietic cell surface of patients with IRH/IRP results in antihuman IgG antibodies generation. The heterogeneity of HI is because humoral and cellular immune activation in different forms.关键词
免疫相关性血细胞减少/造血细胞岛/MHC分子/HLA-DRKey words
IRH/IRP/Hematopoietic island/MHC molecules/HLA-DR分类
医药卫生引用本文复制引用
孙黎飞,韩冰,李钢,吴强强,郝红峰,明汇,张金标..骨髓造血细胞岛在免疫相关性血细胞减少患者中的异质性研究[J].中国免疫学杂志,2012,28(5):458-461,464,5.基金项目
本文为济南军区"十二五"重点支持项目(JN11LO17) （JN11LO17）
