中国免疫学杂志2012，Vol.28Issue(6)：483-488,6.DOI:10.3969/j.issn.1000-484X.2012.06.001
中国恒河猴感染SIV后T淋巴细胞初始、记忆亚群的变化
Dynamics of T-cell memory subtypes in SIV infected Chinese rhesus monkeys
摘要
Abstract
Objective:To explore the dynamics of T-cell naive/memory subsets in SIV infected Chinese rhesus monkeys. Methods: 20 Chinese rhesus monkeys were infected by SIVmac239, and their plasma viral load were determined by quantitative Taqman real-time RT-PCR analysis within a follow-up period of 18 months. Moreover, proportions of their CD4+ T cells and its naive/memory subsets were analyzed by flow cytometry using a combination of surface markers CD3/CD4/CD28/CD95, by which the absolute cell counts of CD4 and its subsets were also computed based on the routine blood tests. Results: Dynamics of the T-cell naive/memory subsets including their percentages and absolute counts were illustrated. Moreover, central memory CD4 showed to be the majority of cell-loss after SIV infection, which decreased more significantly and accordantly than total CD4, and should be further evaluated as a better indicator for clinical surveillance and drugs judgement. Naive CD4 T cell also decreased gradually with disease progression in half of these animals with more variation among individuals. But impressively, 1 monkey ( RM449 ) with rapidly disease progression died with relatively higher naive and central memory CD4 + T cells, which may be partially explained by the blockage of naive CD4 differentiation into effector memory CD4, as revealed by the flow cytometry assay. Conclusion: The dynamics of different subsets of CD4 T cells in Chinese rhesus monkeys infected with SIVmac239 were surveyed. These data are necessary for the further research of cellular anti-SIV immunity, and may be useful for the clinical judgement of immune reconstitution and vaccine evaluation in HIV/AIDS research.关键词
猴免疫缺陷病毒/中国恒河猴/T细胞/记忆细胞亚群Key words
Simian immunodeficiency virus ( SIV )/ Chinese rhesus monkey/ T-cell/ Memory subtypes分类
医药卫生引用本文复制引用
陈颂,鲍琳琳,卢葳,吴小闲,卢耀增,潘菊华,王阶,黄世敬,赖春辉,郭卫中,孙丽华..中国恒河猴感染SIV后T淋巴细胞初始、记忆亚群的变化[J].中国免疫学杂志,2012,28(6):483-488,6.基金项目
本文受国家科技重大专项(No.2008ZX10005-004)项目资助 （No.2008ZX10005-004）
