中国免疫学杂志2012，Vol.28Issue(6)：502-506,5.DOI:10.3969/j.issn.1000-484X.2012.06.005
HIF-1α、MT、Slug和E-cadherin在人乳腺癌中的表达及其意义
The expressions and significances of HIF-1α, MT, Slug and E-cadherin in human breast cancer
摘要
Abstract
Objective:To investigate the expressions and significances of HIF-1 α, MT, Slug and E-cadherin in human breast cancer. Methods: Immunohistochemistry was used to analyze the expression of HIF-1 α, MT, Slug and E-cadherin in 69 tissue samples of breast cancer patients without any radiotherapy, chemotherapy and hormone therapy. The mRNA expressions of HIF-1 α., MT, Slug and E-cadherin in breast cancer cell lines MCF-7 and MDA-MB-231 were detected by RT-PCR analysis. Results: In 69 tissue samples of breast cancer, the positive expression rates of HIF-lα, MT, Slug and E-cadherin were 71. 01% , 42. 03% , 43. 48% and 50. 72%. The expressions of HIF-1 α, Slug and E-cadherin have significant correlations with TNM stage and axillary lymph node metastasis ( P <0. 05 ), the expression of MT has a significant correlation with TNM stage ( P < 0. 05 ). In 69 tissue samples of breast cancer, the expression of HIF-1 α has a positive correlation with that of MT ( r =0. 285,P <0. 05 ), the expressions of MT and Slug were significantly positively correlated ( r =0. 379, P <0. 01 ), and the expression of Slug was significantly negatively correlated with that of E-cadherin ( r=- 0. 422,P <0.01 ) in breast cancer. RT-PCR analysis showed that HIF-lct, MT, Slug and E-cadherin mRNA were expressed in breast cancer cell lines MCF-7 and MDA-MB-231. The mRNA expression levels of HIF-lα, MT and Slug was higher and the expression levels of E-cadherin was lower in MDA-MB-231 cell line than in MCF-7 cell line( P<0.05 ). Conclusion:The expressions of HIF-1 α., MT, Slug and E-cadherin might be used as markers for development of breast cancer.关键词
乳腺癌/HIF-1α/MT/Slug/E-cadherinKey words
Breast cancer/ HIF-1 α/ MT/ Slug/ E-cadherin分类
医药卫生引用本文复制引用
龙方懿,印国兵,刘智敏,杨俊艳,贾朝莉,刘小花,董超然,王旎..HIF-1α、MT、Slug和E-cadherin在人乳腺癌中的表达及其意义[J].中国免疫学杂志,2012,28(6):502-506,5.基金项目
本文受国家自然科学基金面上项目(81072183)、重庆市自然科学基金重点项目(CSTC,2011BA5038)和重庆医科大学重点科研课题(XBZD201002)资助 （81072183）
