中国免疫学杂志2012，Vol.28Issue(6)：507-511,5.DOI:10.3969/j.issn.1000-484X.2012.06.006
白血病细胞系NK细胞配体表达及其对NK-92细胞杀伤敏感性的研究
Research of the expression of NK ligands in leukemia cell lines and their sensitivity to NK-92
摘要
Abstract
Objective: To detect the expression of NK ligands in leukemia cell lines and their sensitivity to NK-92 respectively. And analyze the association between NK ligands' expression and NK cytotoxicity. Methods:Using (3-actin as control gene, 10 NK ligands ( MICA, MICB, ULBP1, PVR, Nectin-2, LFA-3, LLT-1, HLA-E, HLA-F, HLA-G) in 7 leukemia ceU lines ( U937, CEM, KG-la, HL-60, NB4, Reh, LCL) respectively were detected by semi-quantitative RT-PCR method. With CFSE and PI double staining, the NK-92 cytotoxicity against the cell lines were analyzed by flow cytometry. Results: Leukemia cell lines were classified into three groups ( sensitive, moderate and insensitive ) according to their sensitivity to NK-92 cytotoxicity. U937, CEM and KG-1 a were sensitive group, HL-60 and NB4 were moderate group, Reh and LcL were insensitive group. The transcriptional levels of PVR and HLA-F were statistically different among groups ( P value was 0.017 and 0. 016 respectively ). PVR of insensitive group was the lowest while HLA-F of which was the highest. Conclusion: Our study preliminary show that PVR and HLA-F are the potential targets through which we could highly increase the NK effectors in adoptive immunotherapy关键词
白血病/自然杀伤细胞/配体/受体Key words
Leukemia/ Natural killer cell/ Ligand/ Receptor分类
医药卫生引用本文复制引用
罗渊,索塔林,李燕,葛淑静,周洪哲,唐捷,段连宁..白血病细胞系NK细胞配体表达及其对NK-92细胞杀伤敏感性的研究[J].中国免疫学杂志,2012,28(6):507-511,5.基金项目
本文受国家自然科学基金项目(No.30873014)资助 （No.30873014）
