中国免疫学杂志2012，Vol.28Issue(6)：525-529,5.DOI:10.3969/j.issn.1000-484X.2012.06.010
IL-2协同抗CD45RB抗体调节Treg/Th17细胞的比例并延长小鼠移植皮肤存活时间
IL-2 synergy CD45RB mAbs regulate the ratio of Treg/Th17 cells and prolong the survival times of the skin allografts in mice
摘要
Abstract
Objective: To investigate the role of IL-2 on the differentiation of Treg/Thl7 cells in anti-CD45RB mAbs inducing immune tolerance with anti-CD45RB mAbs, and elaborate the mechanism of tolerance induction by anti-CD45RB mAbs further. Methods :CD4+ T cells were harvested from the spleen of C57BL/6 mice by immune magnetic beads and cultured in the present of anti-CD45RB mAbs and IL-2 for 72 h and the ratio of Treg/Thl7 cells were analyzed by flow cytometry. In vivo, BALB/c mice as donor, C57BL/6 mice as recipient to establish skin transplantation model, recipients were received anti-CD45RB mAbs and IL-2 treatment. Splenocytes were harvested on postoperative 1,3,5,7,9 days for dynamic testing Treg/Thl7 cells. On postoperative day 9, the skin allografts infiltrated by inflammatory cells were observed by HE stain; skin grafts survival time were observed and recorded for survival studies. Results: CD4 + T cells cultrued with IL-2 in the present of anti-CD45RB mAbs for 72 h, the ratio of Treg was enhanced and Thl7 was reduced; mice combined treatment of IL-2 and anti-CD45RB mAbs can remarkably prolonged the survival time of skin grafts. Conclusion: IL-2 can significantly enhance anti-CD45RB mAbs induce immune tolerance through up-regulate Treg cells and down-regulate Thl7 cells.关键词
免疫耐受/CD45RB/IL-2/Treg细胞/Th17细胞Key words
Immunce tolerane/ CD4RB/ IL-2/ Treg/ Thl7分类
医药卫生引用本文复制引用
郭伟坚,简优强,张国超,邓春艳,齐晖,李富荣,周汉新..IL-2协同抗CD45RB抗体调节Treg/Th17细胞的比例并延长小鼠移植皮肤存活时间[J].中国免疫学杂志,2012,28(6):525-529,5.基金项目
本文受国家自然科学基金(No.30772042)资助 （No.30772042）
