中国免疫学杂志2012，Vol.28Issue(6)：544-547,4.DOI:10.3969/j.issn.1000-484X.2012.06.014
肿瘤坏死因子-β对重型再生障碍性贫血患者效应T细胞损伤骨髓造血的影响
Effects of TNF-β on damage of hematopoiesis by CD8+HLA-DR+T cells of SAA patients
摘要
Abstract
Objective:To explore the effects of tumor necrosis factor-β( TNF-β )on damage of hematopoietic cells by CD8 + HLA-DR + effector T cells of the patients with severe aplastic anemia( SAA ), and to prove the role of TNF-β in immunopathogenesis of SAA. Methods: CD8 + HLA-DR + T cells as effector cells were sorted from bone marrow mononuclear cells( BMMNC ) of SAA patients by magnetic activated cell sorting system ( MACS ). CD3 + cells MACS depleted BMMNC of normal controls as target cells were co-cultured with the effector cells( 1: 1 ) in different concentrations of TNF-β( 0, 15, 25 and 50 ng/ml ) for 72 hours. The percentage of An-nexin V positive cells were measured by flow cytometry. The quantitive assay of IL-10, IFN-7 in the culture supernatant was also performed using the enzyme linked immunosorbent assay kit. Results: The apoptotic rate( AR ) of target cells in control group and experimental groups were( 49. 85 ± 20. 33 )% ,( 51. 65 ± 20.34 )% ,( 55. 94 ± 20. 19 )% and ( 57.72 ± 19.45 )% . AR of target ceUs in 25 ng/ml and 50 ng/ml TNF-β groups were significiantly higher than those in control group and 15 ng/ml TNF-β group( P <0. 05 ). There were no significiant difference between those in control group and 15 ng/ml TNF-β group, also between those in 50 ng/ml and 25 ng/ ml TNF-β groups( all P >0. 05 ). The levels of IL-10 in the culture supernatant in different groups were ( 217. 99 ± 29. 47 )ng/ml, (216.47 ±29.00 )ng/ml,( 212. 15 ± 13. 19 )ng/ml and (218. 01 ±28.61 )ng/ml . There were no difference among them( P >0. 05 ). The levels of IFN-7 in the culture supernatant of 50 ng/ml TNF-β group[ ( 593. 50 ±48. 18 )ng/ml ] was significiantly higher than those of control group [ ( 544. 85 ± 69. 77 ) ng/ml ], 15 ng/ml TNF-β group [ ( 567. 38 ± 74. 51 )ng/ml ] and 25 ng/ml TNF-(3 group [ ( 544. 05 ±79. 69 )ng/ml ] ( P <0. 05 ). Conclusion:TNF-β could enhance the apoptosis of hematopoietic cells induced by effector T cells of SAA patients in dose-respanse manner. High level of TNF-β might also promote CD8 + HLA-DR+ T cell secrete IFN-7 which could collaborative by damage hematopoiesis.关键词
贫血,再生障碍性/效应T细胞/TNF-βKey words
Anemia, Aplastic/ Effector T cell/ Tumor necrosis factor-β分类
医药卫生引用本文复制引用
刘晓,王晓明,刘鸿,吴玉红,宋嘉,邢莉民,关晶,李丽娟,邵宗鸿,付蓉,王珺 ,王化泉,刘春燕,阮二宝,瞿文,梁勇,王国锦..肿瘤坏死因子-β对重型再生障碍性贫血患者效应T细胞损伤骨髓造血的影响[J].中国免疫学杂志,2012,28(6):544-547,4.基金项目
本文为国家自然科学基金(30971286,30971285)、高等学校博士学科点专项科研基金(200800620004)、天津市应用基础及前沿技术研究计划(08JCYBJC07800,09JCYBJC11200)、卫生部卫生行业科研专项(201002024)、天津市卫生局科技攻关项目(11KG135) （30971286,30971285）
