中国免疫学杂志2012，Vol.28Issue(6)：548-551,4.DOI:10.3969/j.issn.1000-484X.2012.06.015
Poly I: C刺激下原发性干燥综合征患者单核细胞分泌Ⅰ型干扰素能力增强
Poly I:C induced more IFN-α and IFN-β production in monocytes from primary Sj(o)gren Syndrome
摘要
Abstract
Objective:To investigate the production of IFN-α and IFN-β by monocytes from primary Sjogren Syndrome ( pSS ) patients in response to Poly I:C. Methods: The monocytes from pSS patients and healthy controls were treated with 50 μg/ml or 100 μg/ml Poly I:C. The IFN-α and IFN-β level in medium culture was determined by ELISA after stimulated 6 and 12 hours. In the presence or absence of the serum from pSS patients and healthy individuals, we treated the monocytes with 50 μg/ml Poly I: C 12 hours,and then determined the concentration of IFN-α and IFN-β in medium culture. Results:The production of IFN-α and IFN-β by monocytes from pSS patients were induced time and dose dependently by Poly I:C simulation. Increased IFN-α and IFN-β production was observed in monocytes from pSS patients. The serum from pSS patients could enhance the production of IFN-α and IFN-β triggered by Poly I:C. Conclusion: Monocytes from patients with pSS appear more sensitive to Poly I:C, resulting in increased IFN-α and IFN-β production, which could be enhanced by the patients'serum. This abnormality may be a typical characteristic of the pathogenesis of pSS.关键词
干燥综合征/天然免疫/Poly I:C/Ⅰ型干扰素Key words
Sjogren Syndrome/ Innate immunity/ Poly I:C/ Type I interferon分类
医药卫生引用本文复制引用
孙懿,胡志德,谷明莉,黄元兰,薛苗,郭方宇,邓安梅..Poly I: C刺激下原发性干燥综合征患者单核细胞分泌Ⅰ型干扰素能力增强[J].中国免疫学杂志,2012,28(6):548-551,4.基金项目
本文受国家自然科学基金(30972730)和上海市科委基金(09JC1405400)资助 （30972730）
