农学学报2012,Vol.2Issue(3):31-35,5.
非洲菊的组织培养研究
Tissue Culture of Gerbera jamesonii
摘要
Abstract
To provide the basis of the materials for Gerbera’s chemical inducement;and the corresponding set of technique.Using gerbera varieties young receptacle of‘Linda’as explants to tissue culture.the best sterilization time on explants were washed for one hour and sterilized with 75% ethyl alcohol for 30 s,0.1% HgCl2 for 12 minutes,the calluses introduction medium for in vitro culture was MS(murashige and skoog) with 6-BA(5.0 mg/L) + NAA(0.5 mg/L) + sucrose(3%) + agar(7%),the multiplication medium was MS with 6-BA(1.0 mg/L)+NAA(0.1mg/L) + sucrose(3%)+agar(7%),the rooting medium was 1/2MS with NAA(0.1 mg/L)+ sucrose(2%)+agar(7%).From the experiments,it was evident that young receptacle as explants was more easy to surface sterilization,callus induction and multiple shoot clumps,then established rapid propagation system of Gerbera.关键词
非洲菊/组织培养/花托Key words
Gerbera/Tissue Culture/Receptacle分类
农业科技引用本文复制引用
杨尧,任雪,杜兴翠,章四庆,郭巧会,赖齐贤..非洲菊的组织培养研究[J].农学学报,2012,2(3):31-35,5.基金项目
浙江省级创新项目“特殊空间绿化材料新优草花的引种与评价” ()