中国脑血管病杂志2012，Vol.9Issue(5)：259-264,6.DOI:10.3969/j.issn.1672-5921.2012.05.008
应用同步辐射血管造影观察褪黑素对大鼠蛛网膜下腔出血致血管痉挛的影响
Observation of the effect of melatonin on subarachnoid hemorrhage-induced cerebral vasospasm in rats using synchrotron radiation angiography
摘要
Abstract
objective To investigate the effect of melatonin on sub arachnoid hemorrhage ( SAH ) -induced cerebral vasospasm. Methods Sixty SD rats were randomly allocated into a normal control group, a SAH group, a solvent group, and a melatonin group. A rat model of SAH was inducted by injecting autologous blood into prechiasmatic cistern. The melatonin was injected int rape rit one ally once ( 15 mg/ kg) in the rats of melatonin group immediately after modeling, and the same amount of drug was given a-gain at 24 hours. Melatonin was alternated by the equal volume of 10% ethanol ( 15 mL/kg ) in the solvent group, the others management were the same with the melatonin group. The normal control group did not give any treatment. Neurological score was assessed 2 days after surgery; synchrotron radiation angiography ( SRA ) and pathological examination ( HE staining ) were used to observe in vivo rat intracranial vascular changes and the diameter of anterior cerebral artery ( ACA ) at day 3. Results ①The neurobehavioral score in the melatonin group was 15. 3 ± 2. 9, it was higher than 11.8 ±2.3 in the SAH group and 11.6 ± 3.4 in the solvent group ( all P < 0. 05 ), however, they were all lower than 18 in the normal control group ( P < 0. 05 ). ②The ACA diameter was measured by SRA technique, the melatonin group was 304 ± 60μm. There was significant difference as compared to the 215 ±36 μm in the SAH group and 211 ±40 μm in the solvent group ( P < 0. 05 ), however, they still lower than 349 ± 26 μm in the normal control group ( P < 0. 05 ). ③ Pathology measurements of the AC A diameter in the melatonin group was 185 ±34 μm, in the SAH group was 101 ±20 μm, and in the solvent group was 103 ± 19 (xm. There were significant differences ( P<0.05 ); However they were all lower than 216 ± 10 μm in the normal control group ( P <0. 05 ). Conclusion Melatonin may significantly alleviate SAH-induced cerebral vasospasm. SRA is a novel technique that may directly evaluate the SAH-induced cerebral vasospasm of rats in vivo.关键词
蛛网膜下腔出血/血管痉挛,颅内/褪黑素/同步辐射血管造影/大鼠Key words
Subarachnoid hemorrhage/ Vasospasm, intracranial/ Melatonin/ Synchrotron radiation angiography/ Rats引用本文复制引用
陈路佳,蔡军,赵京晶,姜立先,陈左权,凌锋..应用同步辐射血管造影观察褪黑素对大鼠蛛网膜下腔出血致血管痉挛的影响[J].中国脑血管病杂志,2012,9(5):259-264,6.基金项目
国家重点基础研究发展计划(973计划)基金资助项目(2010CB834300) （973计划）
