中国农业大学学报2011，Vol.16Issue(6)：76-82,7.
珠眉海棠（Malus zumi Mats）盐应答MzDREBb基因的克隆与功能研究
Cloning and characterization of salt-responding MzDREBb gene from Malus zumi Mats
摘要
Abstract
The DREBs are transcription factors which play central roles in abiotic stress response.Malus zumi,an apple rootstock,can survive 0.6% NaCl.The full-length cDNA sequence of MzDREBb gene was isolated from a cDNA library of Malus zumi by microarray analysis.The full-length cDNA of MzDREBb was 1 190 bp.Its open reading frame of 714 bp encoded a protein of 237 amino acids.The 5＇-UTR and the 3＇-UTR were 121 and 355 bp respectively.Base on multiple sequence alignment and phylogenetic characterization,MzDREBb was classified into A-5 subgroup of the DREB subfamily.The MzDREBb was localized in the nucleus,which exhibited transactivation activity in yeast two-hybrid system.MzDREBb was induced by either stress of high salinity or low temperature,but not by abscisic acid（ABA）.Transgenic Arabidopsis over-expressing MzDREBb could enhance salt tolerance.In conclusion,MzDREBb is involved in salt response through an ABA-independent pathway in Malus zumi.关键词
珠眉海棠/DREB转录因子/盐胁迫/功能研究Key words
Malus zumi Mats/MzDREBb/salt stress/functional research分类
农业科技引用本文复制引用
黄黎芳,李擎天,蒋玉妆,陈浩,孙扬吾,孔瑾..珠眉海棠（Malus zumi Mats）盐应答MzDREBb基因的克隆与功能研究[J].中国农业大学学报,2011,16(6):76-82,7.基金项目
国家自然科学基金项目(30600416,31171940) （30600416,31171940）
北京市自然科学基金项目(5072027,6112012) （5072027,6112012）
国家“973”计划项目(SQ2011CB016411) （SQ2011CB016411）
