中国农业大学学报2011，Vol.16Issue(6)：137-143,7.
人工感染法氏囊病病毒后鸡法氏囊组织变化的研究
Study on histology impact of chickens＇ bursa of fabricius after infection with infectious bursal disease virus by mark form
摘要
Abstract
To establish the animal model of infectious bursal disease and provide the data base and basic theories of this diseases,5 weeks old SPF chickens were infected by a IBDV strain BC6/85,histolpathological and Immunohistochemistrical methods were used to study the change of the pathological lesions and IBDV immunoreactive positive cells in bursa of fabricius from 2 to 432 h after infection.Results showed that IBDV and pathological lesion could be observed in the chamber and follicles of the bursa of fabricius in infected chickens and the IBDV detected after lesions appearing.Both of the pathological lesion and IBDV immunoreactive positive cells in bursa of fabricius increased at the beginning and then became different because of the positive cells disapeared with the disapearances of the bursal follicles.IBDV and bursal follicles still can be detected in the busal of infected chickens in the later period of infection.The study detail recorded the whole phases of the disease of the chickens infacted IBDV strain BC6/85 and installed the preliminary animal model.Then the form of marking was used to analyze the histological bursa lesions together with the density of IBDV immunoreactive positive cells,and searched mechanism of pathogenic关键词
鸡/法氏囊病病毒/HE染色/免疫组织化学Key words
infectious bursal disease virus/HE staining/immunohistochemistry分类
农业科技引用本文复制引用
俞英昉,王安如,张爽,宋路莎,韩德平,马海燕,张涛,滕可导..人工感染法氏囊病病毒后鸡法氏囊组织变化的研究[J].中国农业大学学报,2011,16(6):137-143,7.基金项目
国家“十一五”科技支撑计划(2008BADB4807) （2008BADB4807）
国家自然科学基金资助项目(30771591,30771566) （30771591,30771566）
北京市自然科学基金资助项目(6082007) （6082007）
