中国农业大学学报2012，Vol.17Issue(2)：90-93,4.
葡萄卷叶伴随病毒1号河北和辽宁分离物CP基因序列分析
Sequences analysis of CP genes in two isolates of Grapevine leafroll-associated virus1 from Hebei and Liaoning Province
摘要
Abstract
In order to improve the detection reliability,grapevine leaves with typical leafroll symptom originated from Huailai region in Hebei and Xingcheng region in Liaoning Province were detected for GLRaV-1 by RT-PCR.The complete sequences of coat protein（CP） gene of GLRaV-1-HB and GLRaV-1-LN isolates were amplified.Sequence comparisons revealed that GLRaV-1-HB and GLRaV-1-LN shared 90.7% and 93.8% identities at nucleotide and amino acid levels,respectively.In contrast with the reported 3 foreign isolates,the identities of nucleotide sequence and deduced amino acid sequence ranged from 90.0% to 96.0% and 92.9% to 97.5%,respectively.The phylogenetic tree from alignments of CP nucleotide sequences of 5 GLRaV-1 isolates revealed the existence of three well-defined clusters.GLRaV-1-HB showed a close relationship with Isolate PS from Brazil（GLRaV-1-PS,GenBank accession number GQ332536.1）,while GLRaV-1-LN and Isolate IR-S7 from Iran（GLRaV-1-IR-S7,FJ952151.2）clustered together.An Australia Isolate（GLRaV-1-AUS,AF195822.1）was grouped into a cluster alone.关键词
葡萄卷叶病/RT-PCR/进化树Key words
grapevine leafroll disease/RT-PCR/phylogenetic tree分类
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刘命华,王克双,齐洪华,吕睦頔,李捷,程玉琴,范在丰..葡萄卷叶伴随病毒1号河北和辽宁分离物CP基因序列分析[J].中国农业大学学报,2012,17(2):90-93,4.基金项目
现代农业产业技术体系建设专项 （）
