中国农业科学2012，Vol.45Issue(5)：832-839,8.DOI:10.3864/j.issn.0578-1752.2012.05.002
紫色马铃薯查尔酮合成酶基因(CHS)的克隆及分析
Cloning and Expression Analysis of a Chalcone Synthase (CHS)Gene from Purple Potato (Solanum tuberosum)
摘要
Abstract
[Objective] The aim of this study was to clone the full length cDNA of chalcone synthase from purple potato, to analyze its expression at different organs and the relationship between CHS expression and anthocyanin accumulation after treated with different concentrations of GA3 and sucrose. [ Method ] The full-length cDN A of CHS was isolated from Solatium tuberosum by RT-PCR and RACE. Semi-quantitative RT-PCR was used to analyze the expression levels of StCHS in different organs, and the expression levels induced by GA3 and sucrose were adopted by semi-quantitative RT-PCR. The anthocyanin content of purple potato was measured by spectrophotometer method. [Result] The cloned full-length CHS was 1 490 bp in length, containing a 1 170 bp open reading frame (ORF), which encodes 389 amino acids. The deduced amino acids contained Cys164, Phe215, His303, and Asn336 active sites, which constitute the catalytic center of CHS. CHS is expressed at different levels in different organs, which was found to be expressed in stems, leaf stalks and leaves but not in roots, tubers or rachises. The anthocyanin accumulation was slightly induced by GA3 as well as the expression of CHS. The anthocyanin accumulation was strongly induced by sucrose, but the CHS expression was not notably induced. [Conclusion] CHS gene was cloned from purple potato and its expression is tissue-specific. StCHS is a rate-limited gene in the flavonoid synthesis pathway of purple potato.关键词
紫色马铃薯/查耳酮合成酶基因/花青素/赤霉素/蔗糖/基因表达Key words
purple potato (Solanum tuberosum)/ chalcone synthase gene (CHS)/ anthocyanin/ GA3/ sucrose/ gene expression引用本文复制引用
胡朝阳,周友凤,龚一富,金思,王何瑜,赵群芬..紫色马铃薯查尔酮合成酶基因(CHS)的克隆及分析[J].中国农业科学,2012,45(5):832-839,8.基金项目
宁波市农业创新创业资金(2010C91050)、宁波市农业科技攻关(2010C10051,2010C10057)、宁波大学学科项目(XKL121,XKL11D2099)、宁波市科技林特与食品加工科技特派团队项目 （2010C91050）
