中国农业科学2012，Vol.45Issue(5)：848-853,6.DOI:10.3864/j.issn.0578-1752.2012.05.004
异三聚体G蛋白在UV-B诱导拟南芥气孔关闭中的作用
Role of Heterotrimeric G Protein in UV-B-Induced Arabidopsis Stomatal Closure
摘要
Abstract
[Objective] The role of heterotrimeric G protein in UV-B-induced stomatal closure was analyzed to provide valuable evidence for further elucidating the mechanism of UV-B signaling transduction in plants. [Method] By using Arabidopsis (Arabidopsis thaliana) wild type, Ga-subunit-knockout mutants gpal-1 and gpal-2, wGa (a gpal overexpression line) and cGa (overexpression constitutively active form mutant of GPA1) combined with pharmacological analysis, the role of heterotrimeric G protein in 0.5 Wm-2 UV-B-induced stomatal closure was explored via stomatal bioassay. [Result] Pharmacological results showed that pertussis toxin (PTX), an inhibitor of G protein a subunit, inhibited UV-B-induced stomatal closure significantly. Meanwhile, cholera toxin (CTX), an activator of G protein a subunit, induced stomatal closure of leaves under light as well as UV-B radiation. Genetic data showed that UV-B-induced stomatal closure was completely impaired in the mutants gpal-1 and gpal-2, but not in the overexpression lines of wGa and cGa. In contrast, cGa showed not only smaller stomatal aperture than wild type under light, but also fester stomatal closure induced by UV-B than they did in the wild type. [Conclusion] Results of this study strongly suggest the involvement of heterotrimeric G protein a subunit in the signaling transduction pathway of UV-B-induced stomatal closure in Arabidopsis.关键词
异三聚体G蛋白/紫外线B辐射/气孔运动/拟南芥Key words
heterotrimeric G protein/ UV-B radiation/ stomatal movement/ Arabidopsis引用本文复制引用
马鲜歌,贺军民..异三聚体G蛋白在UV-B诱导拟南芥气孔关闭中的作用[J].中国农业科学,2012,45(5):848-853,6.基金项目
国家自然科学基金项目(31170370)、中央高校基本科研业务费项目(GK200901013) （31170370）
