中国农业科学2012，Vol.45Issue(5)：973-980,8.DOI:10.3864/j.issn.0578-1752.2012.05.019
蜜蜂患白垩病虫体内一株球囊菌拮抗细菌的分离与鉴定
Isolation and Identification of an Antagonistic Bacterial Strain Against Ascosphaera apis from Honeybee Larvae Infected with Chalkbrood Disease
摘要
Abstract
[Objective] The objective of this study is to explore Ascosphaera apis antagonistic bacteria for biological control of honeybee chalkbrood disease. [Method] An antagonistic bacterial strain was isolated from honeybee larvae suffered from chalkbrood disease on PDA medium plate. It was identified by combining of morphology, gram stain and 16S rDNA sequence analysis. Its inhibiting activity to Ascosphaera apis was determined in vitro and in vivo. [Result] The bacterium was identified as Bacillus cereus. It could inhibit the growth of Ascosphaera apis in PDA medium, but its fermentation solution could not. The death rate and development of the honeybee larvae was unchanged when feeding diet containing the bacteria suggesting that the strain was not pathogenic to honeybee. By inoculating together with the spore of Ascosphaera apis to honeybee larvae, the bacterium could not inhibit the growth of Ascosphaera apis at early infecting stage, but could effectively degrade the hyphae soon after. Ascosphaera apis could not form the offspring spore, thus lost the capability of spreading. [Conclusion] This study provides a theoretical basis for biological control of honeybee chalkbrood disease.关键词
白垩病/蜜蜂球囊菌/蜡样芽孢杆菌/拮抗/分离Key words
chalkbrood disease/ Ascosphaera apis/ Bacillus cereus/ antagonism/ isolation引用本文复制引用
李江红,郑志阳,洪双燕,齐香凤,梁勤..蜜蜂患白垩病虫体内一株球囊菌拮抗细菌的分离与鉴定[J].中国农业科学,2012,45(5):973-980,8.基金项目
国家自然科学基金项目(30600454)、国家蜂产业技术体系建设专项资金(CARS-45-KXJ7)、福建省自然科学基金项目(2010J01060)、国家大学生创新性实验项目(101038918) （30600454）
