中国农业科学2012，Vol.45Issue(5)：990-998,9.DOI:10.3864/j.issn.0578-1752.2012.05.021
鸡突变型和未突变型Mx蛋白体外抗病毒活性研究
Comparison of the In vitro Antiviral Activity Between the Chicken Mutant-Type and Wild-Type Mx Proteins
摘要
Abstract
[Objective] This experiment was conducted to assess the relationship between the 631 amino acid variations of chicken Mx protein and the disease resistance. [ Method] Mutant pcDNA3.0-MMx and wild-type pcDNA3.0-Mx plasmids of Mx protein gene of Chinese Langshan chicken were constructed successfully, then the CEF and NIH-3T3 cells were transfected by mutant pcDNA3.0-MMx and wild-type pcDNA3.0-Mx plasmids, respectively. RT-PCR was used to identify the expression of mutant MMx and wild-type Mx genes and the mini-cytopathic effect inhibition assay was used to evaluate the antiviral activity for Newcastle disease virus (NDV) and vesicular stomatitis virus (VSV). [ Result] The recombinant Mx protein protected the CEF cells from NDV infection up to 48-h incubation while the NIH-3T3 cells transfected by pcDNA3.0-MMx were not infected by 60-h incubation with VSV, however, the CEF and NIH-3T3 cells transfected by pcDNA3.0-Mx developed pathological changes during 24-h incubation with the viruses. [Conclusion] Recombinant Mx protein could delay the infection of NDV and VSV at single-cell level.关键词
Mx蛋白/抗病毒活性/抗NDV/抗VSV/狼山鸡Key words
Mx protein/ antiviral activity/ NDV/ VSV/ Langshan chicken引用本文复制引用
傅德智,孙敏,郑蒙蒙,高波,张亚妮,窦套存,王克华,李碧春,孙怀昌..鸡突变型和未突变型Mx蛋白体外抗病毒活性研究[J].中国农业科学,2012,45(5):990-998,9.基金项目
国家自然科学基金(30671509,30871791)、江苏省高校自然科学重大基础研究项目(08KJA230001)、江苏省六大人才高峰基金 （30671509,30871791）
