中国普通外科杂志2012，Vol.21Issue(3)：312-316,5.
MicroRNA在人胰腺癌中表达谱的初步研究
Preliminary study of microRNA expression profiles in human pancreatic cancer
郑岩松 1谢荣臻 1石铮1
作者信息
- 1. 福建医科大学附属第一医院肝胆外科,福建福州350005
- 折叠
摘要
Abstract
Objective: To investigate the microRNAs (miRNAs) expression profiles in human pancreatic cancer, so as to provide a basis for further exploring the role of miRNAs in the molecular mechanism that leads to the occurrence and development of pancreatic cancer.Methods: The differentially expressed miRNAs between the pancreatic cancer tissue and adjacent normal pancreatic tissue were analyzed by microRNA array, and then two of the differentially expressed miRNA were selected to perform quantitative real time PCR (qRT-PCR) assay, so as to validate the microRNA array results. Results: Compared with the normal pancreatic tissues, 15 miRNAs were more than 1.5-fold up-regulated, and 11 miRNAs were more than 1.5-fold down-regulated in pancreatic cancer tissues. The values of relative quantification (RQ) of miR-lOb and miR-21 in pancreatic cancer tissues and their adjacent normal tissues from 8 cases detected by qRT-PCR were 0.0743±0.0222 vs. 0.0287±0.0129, and 0.3062±0.1117 vs. 0.0240±0.0137, respectively (both P<0.05), and these were consistent with the microRNA array results. Conclusion: The 26 differentially expressed miRNAs screened by microRNA array may contribute to the occurrence and development of pancreatic cancer,and their respective roles in this disease are worthy of further investigation.关键词
胰腺肿瘤/微小RNA/微阵列分析/聚合酶链反应Key words
Pancreatic Neoplasms/ microRNA/ Microarray Analysis/ Polymerase Chain Reaction分类
医药卫生引用本文复制引用
郑岩松,谢荣臻,石铮..MicroRNA在人胰腺癌中表达谱的初步研究[J].中国普通外科杂志,2012,21(3):312-316,5.
