中国普通外科杂志2012，Vol.21Issue(3)：317-321,5.
RNAi载体抑制survivin基因在胰腺癌PANC-1细胞中的表达
Survivin gene inhibition in pancreatic cancer PANC-Ⅰ cells by RNAi vectors
陈俭云 1崔海宁2
作者信息
- 1. 广东省深圳市第五人民医院(罗湖医院)普通外科,广东深圳518001
- 2. 海南医学院附属医院普通外科,海南海口570102
- 折叠
摘要
Abstract
Objective: To observe the inhibitory effect of survivin specific RNA interference (RNAi) vectors on thesurvivin mRNA and protein expression of pancreatic cancer PANC-1 cells.Methods: The plasmid vectors encoding U6 promoter-driven siRNA against survivin and the negative controlplasmid were transfected into the pancreatic carcinoma PANC-1 cells, respectively. The mRNA and proteinexpression of survivin were detected by RT-PCR and Western blot, respectively.Results: In the PANC-1 cells transfected with RNAi vectors against survivin, the 24 h and 48 h inhibitoryratios on survivin mRNA expression were (52.67± 3.51)% and (75.33±3.06)%, and those on proteinexpression were (58.00±3.61)% and (76.67±4.73)%, respectively. All the differences had statistical significancecompared with the negative control cells (all P<0.05).Conclusion: The RNAi vectors with U6 promoter can effectively inhibit the survivin expression in pancreatic carcinoma PANC-1 cells.关键词
胰腺肿瘤/存活素/RNA干扰Key words
Pancreatic Neoplasms/ Survivin/ RNA Interference分类
医药卫生引用本文复制引用
陈俭云,崔海宁..RNAi载体抑制survivin基因在胰腺癌PANC-1细胞中的表达[J].中国普通外科杂志,2012,21(3):317-321,5.
