中国普通外科杂志2012，Vol.21Issue(4)：421-426,6.
miR-214对胃癌细胞SGC7901侵袭和转移能力的影响
Enhancement effects of miR-214 on invasive and metastatic ability of gasric cancer cell line SGC7901
赵雪琰 1郑长黎1
作者信息
- 1. 中南大学基础医学院病理学系,湖南长沙410013
- 折叠
摘要
Abstract
Objective: To investigate the effect of miR-214 on the invasive and metastatic ability of gastric cancer cells andits possible mechanism.Methods: The potential binding sites of PTEN (phosphatase and tensin homolog deleted on chromosometen) for miR-214 were analyzed at miRNA target-gene prediction websites: miRanda and TarBase v.Sc.The SGC7901 cells were transfected with miR-214 mimic, inhibitor or non-targeting sequence, and cellstransfected with the empty vector were used as negtive control. After the above treatments, the PTEN proteinexpression of the cells was detected by Western blot analysis and the invasive ability of the cells was examinedby Transwell chamber assay.Results: Western blot showed that PTEN protein expression in SGC7901 cells group transfected withmiR-214 mimic was significantly decreased compared with the cells of other treatment groups (all P<0.05).Transwell assay showed that the invading cell numbers of SGC7901 cells group transfected with miR-214mimic were significantly more than those of the cells of other treatment groups (all P<0.05).Conclusion: miR-214 can enhance the invasion and metastasis of gastric cancer cells and this effect may berelated to its action in inhibiting PTEN expression.关键词
胃肿瘤/病理学/microRNA/miR-214/肿瘤侵袭Key words
Stomach Neoplasms/pathol/ microRNA/ miR-214/ Neoplasm Invasiveness分类
医药卫生引用本文复制引用
赵雪琰,郑长黎..miR-214对胃癌细胞SGC7901侵袭和转移能力的影响[J].中国普通外科杂志,2012,21(4):421-426,6.
