红细胞平均体积联合葡萄糖-6-磷酸脱氢酶活性测定在地中海贫血初筛中的价值研究OA北大核心CSCDCSTPCD
Study on Value of Joint Detection of MCV and G6PD Activity in Thalassemia Screening
目的 探讨红细胞参数中红细胞平均体积(MCV)与葡萄糖-6-磷酸脱氢酶(G6PD)活性测定在地中海贫血(地贫)初筛实验中的联合应用价值.方法 采用聚合酶链式反应及反向点杂交技术对229例孕妇的血标本进行基因检测(地贫组163例,对照组66例),采用速率法检测G6PD活性,采用鞘流电阻抗法检测MCV,将单项、两项并联和串联结果与基因诊断(金标准)的地贫结果比较,并进行统计分析.结果 MCV、G6PD、两项并联和两项串联诊断地贫的灵敏度分别为99.4…查看全部>>
Objective To explore the application value of joint detection of MCV and G6PD activity in thalassemia screening. Methods Two hundred and twenty - nine blood samples of pregnant women were detected by PCR and reverse dot - blotting, which were divided into two groups, thalassemia's group: 163 cases of thalassemia, normal control group: 66 cases. Meanwhile, G6PD activity and MCV of them were detected by applying rate method and impedance method. Compared singl…查看全部>>
袁明生;易旺云
528415,广东省中山市陈星海医院检验科528415,广东省中山市陈星海医院妇产科
医药卫生
红细胞平均体积葡萄糖-6-磷酸脱氢酶活性地中海贫血初筛串联并联反向点杂交技术
Mean red cell volume G6PD activity Thalassemia Screening Series connection Parallel connection Reverse blotting
《中国全科医学》 2012 (17)
1948-1950,3
评论