首页|期刊导航|中国神经再生研究（英文版）|Optical control after transfection of channelrhodopsin-2 recombinant adenovirus in visual cortical cells
中国神经再生研究（英文版）2012，Vol.7Issue(16)：1228-1233,6.DOI:10.3969/j.issn.1673-5374.2012.16.004
Optical control after transfection of channelrhodopsin-2 recombinant adenovirus in visual cortical cells
Optical control after transfection of channelrhodopsin-2 recombinant adenovirus in visual cortical cells
摘要
Abstract
Channelrhodopsin-2 ectopically expressed in the retina can recover the response to blue light in genetically blind mice and rats, but is unable to restore visual function due to optic nerve or optic tract lesions. Long Evans rats at postnatal day 1 were used for primary culture of visual cortical cells, and 24 hours later, cells were transfected with recombinant adenovirus carrying channelrhodopsin-2 and green fluorescent protein genes. After 2-4 days of transfection, green fluorescence was visible in the cultured cells. Cells were stimulated with blue light (470 nm), and light-induced action potentials were recorded in patch-clamp experiments. Our findings indicate that channelrhodopsin-2-recombinant adenovirus transfection of primary cultured visual cortical cells can control the production of action potentials via blue light stimulation.关键词
channelrhodopsin-2/recombinant adenovirus/visual cortex/blue light/action potentials/neural re-generationKey words
channelrhodopsin-2/recombinant adenovirus/visual cortex/blue light/action potentials/neural re-generation引用本文复制引用
Junping Yao,Wensheng Hou,Hao Wang,Hui Liu,Chuanhuang Weng,Zhengqin Yin..Optical control after transfection of channelrhodopsin-2 recombinant adenovirus in visual cortical cells[J].中国神经再生研究（英文版）,2012,7(16):1228-1233,6.基金项目
The study was sponsored by the National Natural Science Foundation of China (General Program),No.81070749,31070882,30970758 and Key Project of Chongqing Science & Technology Commission,No.2010AB5118. （General Program）
