中国生物防治学报2012，Vol.28Issue(2)：181-185,5.
莱氏野村菌CQNr01对斜纹夜蛾幼虫的侵染研究
Infection of Nomuraea riley CQNr01 against Larvae of Spodoptera litura
摘要
Abstract
Virulence of Nomuraea riley CQNr01 against the 2^nd and 4^th instar larvae of Spodoptera litura was investigated.Spore germination and appressorium formation on isolated epidermis of S.litura was observed by fluorescence microscopy.Results indicated at the concentration of 1×10^8 and 1×10^9 spores·mL ^-1,the mortality of the 2^nd instar larvae caused by N.riley CQNr01 was 86.58% and 89.91%,respectively,while the mortality of the 4th instar larvae was only 13.33% and 16.67%.Under fluorescence microscopy,spores of CQNr01 were found adhered to isolated epidermis of the 2^nd and the 4th instar larvae,and spores germinated within 24 h post inoculation,while adhesion strength of the 2^nd instar larvae was stronger.The germ tubes grew longer and the germination rate became larger as the inoculation time went on.However,under the same inoculation time,the germination rate and the number of appressorium on the 2^nd instar larvae significantly exceeded to those on the 4^th instar larvae.The study showed that virulence of N.riley CQNr01 against the 2^nd and the 4^th instar larvae was significantly different and the epidermis may be the main reason.关键词
斜纹夜蛾/莱氏野村菌CQNr01/荧光染色/侵染性/生物测定Key words
Spodoptera litura/Nomuraea riley CQNr01/fluorescent staining/pathogenicity/bioassay分类
农业科技引用本文复制引用
于海勇,王中康,张雯迪,殷幼平..莱氏野村菌CQNr01对斜纹夜蛾幼虫的侵染研究[J].中国生物防治学报,2012,28(2):181-185,5.基金项目
国家公益性行业（农业）科研专项 （）
国家“863”计划 （）
