中国生物防治学报2012，Vol.28Issue(2)：198-204,7.
四株对小地老虎有活性的苏云金芽胞杆菌的毒力评价
Toxicity Evaluation of Four Bacillus thuringiensis Strains against Agrotis ypsilon
摘要
Abstract
The types of insecticidal genes and expressed proteins,expressed protein contents and insecticidal activity of four Bacillus thuringiensis strains were evaluated in this study.Results indicated that more types of insecticidal gene and protein were found in the strains PS9-D12 and PS9-C12 and the amount of expressed protein in PS9-D12 and PS9-C12 was 1.7 fold that in HD-1.Bioassay results with crystal-spore powder of the strains prepared against Agrotis ypsilon in the same cultured condition showed that the LC50 value of strains HD-1,PS9-D12,PS9-C12,PS9-D11and PS9-H9 were 0.71,0.19,0.14,0.24 and 1.16 mg· g^- 1,respectively.The virulence of the strains PS9-D12 and PS9-C12 was significantly higher than that of the strain HD-1（P〈0.05）.The toxicity of 4 strains in this study to larvae of Plutella xylostella with mortality of more than 78%,and more virulent to larvae of Spodoptera exigua than that of HD-1,but no insecticidal activity to larvae of Colaphellus bowringi.All results suggest that PS9-D12 and PS9-C12 with higher insecticidal activity than commercial strain HD-1 can be good candidates to develop new B.thuringiensis pesticides.关键词
苏云金芽胞杆菌/小地老虎/微生物杀虫剂/PCR-RFLP/MALDI-TOF-MSKey words
Bacillus thuringiensis/Agrotis ypsilon/pesticides/protein expression分类
农业科技引用本文复制引用
刘晓垒,王品舒,束长龙,王秀丽,郝再彬,张永军,宋福平..四株对小地老虎有活性的苏云金芽胞杆菌的毒力评价[J].中国生物防治学报,2012,28(2):198-204,7.基金项目
国家“973”项目 （）
转基因生物新品种培育科技重大专项 （）
国家“863”计划项目（2011AA10A203）,致谢：感谢中国农业科学院植物保护研究所棉花害虫组陆琼博士在生测方面的无私帮助. （）
