中国生物防治学报2012，Vol.28Issue(2)：226-234,9.
一株银杏内生真菌的鉴定及其活性代谢产物研究
Identification of an Endophytic Fungus of Ginkgo biloba TMSF169 and Its Antifungal Metabolites
摘要
Abstract
Endophytic fungi in medicinal plants are important sources for microorganisms which can produce a variety of bioactive compounds.In this study,an endophytic fungus TMSF169 with antifungal activity was isolated from Ginkgo biloba in Tianmushan Nature Reserve,Zhejiang province.TMSF169 was identified as Penicillium commune based on morphology and ITS rDNA sequence analysis.An antifungal and herbicidal compound TMSF169A was isolated and purified from the fermentation broth of TMSF169 through normal-phase silica gel column chromatography and Sephadex LH-20 gel column chromatography,traced by ultraviolet light or iodine vapor with bioassay-guided fractionation.TMSF169A was elucidated as cyclopaldic acid based on mass spectrometry and nuclear magnetic resonance spectroscopy.Antifungal activity assays showed that the compound had inhibitory to a series of plant pathogenic fungi.The metabolite was also pathogenic to Commelina communis,Digitaria sanguinalis,Amaranthus retroflexus,Alternanthera philoxeroides and Oryza sativa,but not sensitive to Sesamum indicum and Glycine max.All the results demonstrated that the strain TMSF169 deserves to develop further.关键词
内生真菌/团青霉/活性代谢产物/除草活性/圆弧菌醛酸Key words
endophytic fungus/Penicillium commune/active metabolite/herbicide activity/cyclopaldic acid分类
农业科技引用本文复制引用
王国平,王丽薇,张亚磊,王佳莹,徐旭辉,章初龙..一株银杏内生真菌的鉴定及其活性代谢产物研究[J].中国生物防治学报,2012,28(2):226-234,9.基金项目
公益性行业（农业）科研专项 （）
杭州市创业种子基金 （）
