中国生物防治学报2012，Vol.28Issue(2)：235-242,8.
枯草芽孢杆菌B006对黄瓜枯萎病菌和辣椒疫霉病菌的抑制作用及其抗菌组分分析
Antifungal Activities and Principal Component Analysis of Bacillus subtilis B006 against Fusarium oxysporum f. sp. cucumerinum and Phytophthora capsici
摘要
Abstract
Methanol extract from cell-free supernatants of Bacillus subtilis B006 suppressed hyphal growth of Fusarium oxysporunm f.sp.cucumerinum more greatly than that of Phytophthora capsici,while suppressed spore germination of F.oxysporunm f.sp.cucumerimerinum less than that of zoospores of P.capsici.The inhibition was not changed when the extract was heated at 60,90,or 120 ℃ for 10 minutes.TLC analysis of the HCL hydrolysis solution of the extract gave rise to orange spots,indicating that the extract belongs to cyclopeptide.HPLC-ESI-MS analysis showed that the extract contained surfactin and fengycin with m/z at 995,1009,1023,1037,1051 and 1436,1450,1478,1492.When cucumber and pepper were planted in sterile nursery substrate inoculated with B006 at 10^6 cfu· g^-1,surfactin and fengycin could also be detected with HPLC-ESI-MS from SPE-extracted lipopeptides of roots of the plants at 4 to 6 leaf stage,while not from that of uninoculated plant roots.This research indicated that B.subtilis B006 produced lipopeptides,an important group of antibiotics,in plant rhizosphere,suggesting its possible role in biological control of soilborne plant disease in vivo.关键词
脂肽类抗生素/黄瓜枯萎病菌/辣椒疫霉病菌/芽孢杆菌Key words
lipopeptides/Fusarium oxysporum f.sp.cucumerinum/Phytophthora capsici/Bacillus subtilis分类
农业科技引用本文复制引用
杨琦瑶,索雅丽,郭荣君,李世东,许修宏..枯草芽孢杆菌B006对黄瓜枯萎病菌和辣椒疫霉病菌的抑制作用及其抗菌组分分析[J].中国生物防治学报,2012,28(2):235-242,8.基金项目
国家大宗蔬菜产业技术体系建设项目 （）
公益性行业（农业）科研专项 （）
中央级公益性科研院所基本科研业务费专项资金资助项目 （）
