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番茄灰霉病菌拮抗放线菌的筛选、鉴定及其活性评价

徐大勇 李志栋 李峰 徐顶泉 吴旺丽

中国生物防治学报2012,Vol.28Issue(2):298-302,5.
中国生物防治学报2012,Vol.28Issue(2):298-302,5.

番茄灰霉病菌拮抗放线菌的筛选、鉴定及其活性评价

Screening,Identification and Inhibitory Activity of an Actinomycete Strain and against Botrytis cinerea

徐大勇 1李志栋 1李峰 1徐顶泉 1吴旺丽1

作者信息

  • 1. 淮北师范大学生命科学学院/资源植物生物学安徽省重点实验室,淮北235000
  • 折叠

摘要

Abstract

An actinomycete strain HNU-1,isolated from a farmland soil in Shou county,Anhui province,exhibited antifungal activity against Botrytis cinerea.Based on characteristics,in morphology,physiological,biochemica and 16S rRNA gene sequences,the strain was identified as Streptomyces hygroscopicus.Culture filtrate of the strain could inhibit mycelium growth and spore germination of B.cinerea,and resulted in more inhibition as the concentration of the culture filtrate increased.Mycelium growth and spore germination of B.cinerea was completely inhibited by 6.67-fold dilution of the culture filtrate.Pot experiments demonstrated that prevention and cure efficiencies of 6.67-fold dilution of the culture filtrate against tomato gray mould were 87.8% and 77.9%,respectively,significantly superior to those of 500-fold dilution of 50% Badistan.

关键词

番茄灰霉病/灰葡萄孢/吸水链霉菌/鉴定/生物防治

Key words

tomato gray mould/Botrytis cinerea/Streptomyces hygroscopicus/identification/biocontrol

分类

农业科技

引用本文复制引用

徐大勇,李志栋,李峰,徐顶泉,吴旺丽..番茄灰霉病菌拮抗放线菌的筛选、鉴定及其活性评价[J].中国生物防治学报,2012,28(2):298-302,5.

基金项目

资源植物生物学安徽省重点实验室开放课题 ()

安徽高校省级自然科学研究项目 ()

中国生物防治学报

OA北大核心CSCDCSTPCD

2095-039X

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