中国比较医学杂志2012，Vol.22Issue(3)：5-11,7.DOI:10.3969/j.issn.1671.7856.2012.03.002
非酒精性脂肪肝大鼠脂质代谢及病理变化的动态观察
The Dynamic Observation of Lipid Metabolism and Pathological Changes in Non-alcoholic Fatty Liver Rats
摘要
Abstract
Objective To investigate the pathogenesis of nonalcoholic fatty liver disease ( NAFLD) , provide a reference for the evaluation of fatty liver therapeutic effect, the liver function, lipid metabolism, insulin resistance and liver apoptosis of NAFLO rats established by high cholesterol feeding were continuously monitored from 4 - 16 weeks. Methods 40 NAFLD rats established by high cholesterol feeding were randomly divided into the 4,8,12,16 weeks model group and a group of normal rats were set up for control, the serum GLU、CH0 、TG、HDL、LDL、GPT、GOT and insulin were tested, the liver apoptosis and pathological changes were observed, then analyzed the effect of the liver function, lipid metabolism, insulin resistance and apoptosis on the pathological changes in non-alcoholic fatty liver rats. Results After 4 weeks of highcholesterol feeding, the rats had got the liver injury, disorder of lipid metabolism, insulin resistance. Liver cell apoptosis were increased obviously, and pathological changes were characterized by cell steatosis and inflammatory, the lesions became more serious with time went on. Conclusions Rats established by high cholesterol feeding could get the nonalcoholic fatty liver disease and the lesions became more serious with time over during 4-16 weeks. Lipid metabolism disorder and insulin resistance were the important cause of liver cell steatosis and inflammatory. The model we established is suitable for the evaluation of fatty liver therapeutic effect.关键词
非酒精性脂肪肝/胰岛素抵抗/细胞凋亡/病理/脂质代谢Key words
Nonalcoholic fatty liver disease ( NAFLD )/Insulin resistance ( IR )/Apoptosis/Pathology/Lipid metabolism分类
医药卫生引用本文复制引用
楼琦,石巧娟,郭红刚,李巍,卢领群,周文伟,萨晓婴..非酒精性脂肪肝大鼠脂质代谢及病理变化的动态观察[J].中国比较医学杂志,2012,22(3):5-11,7.基金项目
“十一五”科技支撑计划重点项目(2009BAI83B02) （2009BAI83B02）
浙江省医药卫生计划项目(2006QN001,2009A001). （2006QN001,2009A001）
