中国动物传染病学报2012，Vol.20Issue(1)：1-9,9.
猪繁殖与呼吸综合征病毒5′非翻译区中一顺式结构因子对病毒感染必要性的分析
ANALYSIS OF A CIS-ACTING STRUCTURAL ELEMENT IN THE 5＇ UNTRANSLATED ELEMENT OF PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS
摘要
Abstract
It is believed that the 5＇ untranslated region （UTR） of equine arthritis virus plays a critical role in viral genomic replication, subgenomic mRNA transcription and protein translation, yet its structure and function are still largely unknown. We constructed a number of 5＇ UTR truncated mutants based on the full-length cDNA clone pAPRRS of attenuated genotype II Porcine reproductive and respiratory syndrome virus （PPRSV）. Genetic and virologic characteristics of the rescued viruses were analyzed post RNA and DNA transfection. The truncations at the 5＇ UTRs of the revertant viruses were repaired with exogenous nucleotides of unknown origin. Because of difference in the transcriptional initiation sites for T7 and CMV promoters, we introduced a GC-rich sequence into the mutant site in order to investigate if the virus rescue was template dependent. The results showed that the repair mechanism was template independent. Further analysis of the secondary structure demonstrated that the SL1 of PRRSV 5＇ UTR was indispensable for virus infectivity.关键词
非翻译区/茎环结构/回复突变体/RNA／DNA导策Key words
Untranslated region (UTR)/stem-loop (SL)/revertant/RNA/DNA driven分类
农业科技引用本文复制引用
高飞,陆嘉琦,袁世山,童光志..猪繁殖与呼吸综合征病毒5′非翻译区中一顺式结构因子对病毒感染必要性的分析[J].中国动物传染病学报,2012,20(1):1-9,9.基金项目
国家自然科学基金面上项目 （）
欧盟“第七框架”项目 （）
