中国动物传染病学报2012，Vol.20Issue(1)：16-21,6.
一株牛蛙源虹彩病毒的分离及鉴定
CHARACTERIZATION OF AN IRIDOVIRUS ISOLATE FROM RANA CATESBIANA
摘要
Abstract
An iridovims strain F J049 was isolated on epithelioma papillosum cyprinid （EPC） cell line from a moribund American bullfrog （Rana catesbiana） on a farm in Fujian Province. The infected EPC cells developed cytopathogenic effects （CPE）, such as rounding, granulation and detachment. The monoclonal antibody against iridovirus showed positive reaction in the FJ049-infected EPC cells in indirect immunofluorescence assay and specific fluorescence appeared in the cytoplasm of infected cells. A specific 53 lbp fragment was amplified in PCR from the conserved regions of major capsid protein （MCP） gene. The phylogenetic analysis ofMCP gene showed that the FJ049 strain shared 99.8% identity with the RGV9808 strain isolated from Rana grylio stejneger, classified in genus Ranavirus, family Iridoviridae.关键词
鲤鱼表皮瘤细胞系/牛蛙/虹彩病毒/分离/鉴定Key words
Epithelioma papulosum cyprini cell line (EPC)/Rana Catesbiana/Iridovirus/isolation/characterization分类
农业科技引用本文复制引用
刘晓东,杨金先,龚晖,朱春华,林天龙..一株牛蛙源虹彩病毒的分离及鉴定[J].中国动物传染病学报,2012,20(1):16-21,6.基金项目
福建省科技创新平台项目 （）
福建省农业科学院创新项目 （）
