中国动物传染病学报2012，Vol.20Issue(2)：11-16,6.
传染性支气管炎病毒非结构蛋白nsp5在真核细胞中的重组表达及鉴定
EXPRESSION AND CHARACTERIZATION OF RECOMBINANT NON-STRUCTURAL PROTEIN 5 OF INFECTIOUS BRONCHITIS VIRUS
摘要
Abstract
Little is known about the function of non-structural proteins 5（nsp5） of avian infectious bronchitis virus（IBV）.In this study,IBV nsp5 was amplified in RT-PCR using RNA of nephropathogenic IBV strain as template.The recombinant plasmid IBVnsp5-Bacmid was constructed based on Bac-to-Bac baculovirus expression system and transfected into Sf9 cells.The expression of recombinant nsp5 in Sf9 cells was confirmed by IFAT and Western blot analysis.The polyclonal antiserum against nsp5 was prepared using recombinant nsp5 purified from transfected Sf9 cells and demonstrated to react with IBV SC021202 infected DF-1 cells in IFAT.The results indicated that IBV nsp5 was successfully expressed in eukaryotic expression system and had good angenicity.关键词
传染性支气管炎病毒/非结构蛋白5/Bac-to-Bac杆状病毒表达系统Key words
Infectious bronchitis virus/non-structural protein(nsp5)/Bac-to-Bac baculovirus expression system分类
农业科技引用本文复制引用
耿阳,牟小东,刘然,边葶苈,廖敏,周继勇..传染性支气管炎病毒非结构蛋白nsp5在真核细胞中的重组表达及鉴定[J].中国动物传染病学报,2012,20(2):11-16,6.基金项目
现代农业产业技术体系专项经费项目 （）
教育部新世纪人才项目 （）
浙江省教育厅科研项目 （）
