中国动物传染病学报2012，Vol.20Issue(2)：22-28,7.
猪源牛病毒性腹泻病毒SD0803株细胞传代研究
BIOLOGICAL CHARACTERISTICS OF PASSAGED ON MDBK CELLS BOVINE VIRAL DIARRHEA VIRUS OF PIG ORIGIN
摘要
Abstract
To develop a Bovine viral diarrhea virus（BVDV） vaccine,the BVDV strain SD0803 of pig origin was passaged in MDBK cells for 40 times.Viral RNA was extracted from the 40th passage virus and 5 segments were amplified in RT-PCR.The complete genomic sequence was megaligned and compared with its parental virus using DNASTAR software.The results showed that the homology between the 40th passage and parental virus was 99.8% in nucleotides and 99.6 % in amino acids.Twenty three nucleotide mutations were identified,of which 15 were sense mutations.Amino acid mutations were mainly located on E2 and NS5B.To compare the growth characteristics between the parent virus and passaged viruses,supernatants were collected from infected MDCK cells at passages 1,10,20,30 and 40,and measured the amounts of released viral RNAs in RT-PCR.The multi-step growth curves showed that the parent virus and passaged viruses had high replication efficiency in MDBK cells,and shared similar growth properties.Some mutations that occurred during virus passages had no effect on the virus titers as determined by titration.关键词
牛病毒性腹泻病毒/病毒传代/全长测序/Real-timePCR/多步生长曲线Key words
Bovine viral diarrhea virus/virus passage/full-length genome sequencing/Real-time PCR/multi-step growth curve分类
农业科技引用本文复制引用
孙春清,邓宇,张宏彪,龙进学,韦祖樟,童光志,袁世山..猪源牛病毒性腹泻病毒SD0803株细胞传代研究[J].中国动物传染病学报,2012,20(2):22-28,7.基金项目
中央级公益性科研院所基本科研业务费专项资金项目 （）
