中国动物传染病学报2012，Vol.20Issue(2)：29-35,7.
猪繁殖与呼吸综合征病毒ORF5a/ORF5重叠区的分离
THE SEPERATION OF ORF5A/ORF5 OVERLAPPING GENES OF PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRVS
摘要
Abstract
For the first time,a new chimeric Porcine reproductire and respiratory syndrome virus（PRRSV） was constructed by replacing ORF2-5a of the full-length infectious cDNA clone pARRSasc（type 2） with their corresponding counterparts of the clone pSHE（type 1）.This study was designed to（1） make ORF5a independent of ORF5;（2） dissertate the structure and function of 5a protein;（3） elucidate the structure-function relationship of inter-genotypic PRRSV structural proteins.The results showed that ORF2-5a of PRRSV type 1 was stable and expressed structural proteins and played full functions in the backbone type 2 virus.The construction of the chimeric PRRSV expressing ORF5a independent of ORF5 has laid foundation to better understand the structure and function of 5a protein.关键词
猪繁殖与呼吸综合征病毒/嵌合/重叠序列/5a蛋白Key words
Porcine reproductive and respiratory syndrome virus(PRRSV)/chimeric/overlapping genes/5a protein分类
农业科技引用本文复制引用
刘闰夏,蔺涛,田德斌,韦祖樟,孙利厂,陆嘉琦,袁世山,童光志..猪繁殖与呼吸综合征病毒ORF5a/ORF5重叠区的分离[J].中国动物传染病学报,2012,20(2):29-35,7.基金项目
国家自然科学基金面上项目 （）
