中国动物传染病学报2012,Vol.20Issue(2):42-46,5.
鸭坦布苏病毒E蛋白的原核表达
EXPRESSION OF E PROTEIN OF DUCK TEMBUSU VIRUS IN PROKARYOTIC CELLS
摘要
Abstract
The E gene of duck Tembusu virus(DTMUV) was amplified in RT-PCR and inserted into the multiple clone sites of the pET32a(+) vector.The recombinant plasmid was transformed into BL21(DE3) competent cells,and expression of the E protein was induced with IPTG.The E protein was expressed in BL21(DE3) cells and visualized to react with specific DTMUV antibody in SDS-PAGE and Western blot.关键词
鸭坦布苏病毒/E基因/克隆/表达Key words
Duck Tembusu virus/E gene/cloning/expression分类
农业科技引用本文复制引用
姬希文,李雪松,边延峰,李国新,颜丕熙,张七斤,李泽君..鸭坦布苏病毒E蛋白的原核表达[J].中国动物传染病学报,2012,20(2):42-46,5.基金项目
公益性行业(农业)专项经费 ()
国家国际科技合作项目 ()
中国农业科学院上海兽医研究所中央级公益性科研院所基本科研业务费专项资金项目 ()