中国动物传染病学报2012，Vol.20Issue(2)：53-58,6.
pVAX1/SjTSP2 DNA疫苗免疫预防小鼠日本血吸虫病的效果评估
PROTECTIVE EFFICACY OF PVAX1/SJTSP2 AS A DNA VACCINE AGAINST SCHISTOSOMIASIS JAPONICUM IN BALB/C MICE
摘要
Abstract
To evaluate the protective efficacy of pVAX1/SjTSP2 as a DNA vaccine against Schistosomiasis japonicum,a recombinant plasmid pVAX1/SjTSP2 was constructed,and expressed in 293T cells.Purified plasmid was delivered into the mice by particle bombardment with a dose of 2.5μg/mouse.Specific anti-SjTSP2 antibody level were significantly increased in all animals vaccinated with pVAX1/SjTSP2 detected by ELISA in comparison to PBS control group.Splenocytes from mice vaccinated with pVAX1/SjTSP2 produced considerably higher levels of T helper 1 response–enhancing cytokines（IFN-γ） and T helper 2（Th2） response–enhancing cytokines（IL-4） by flow cytometry.The worm burden of vaccinated mice was increased 12.04%（P=0.368）,and liver egg count reduced 10.01%（P=0.486）,compared with PBS control group,respectively.This result may provide another evidence for evaluating SjTSP2 potential as a vaccine candidate.关键词
日本血吸虫/DNA疫苗/SjTSP2/基因枪Key words
Schistosoma japonicum/DNA vaccine/SjTSP2/gene gun分类
农业科技引用本文复制引用
曹燕,赵彬,韩艳辉,艾德宙,张娟,石耀军,洪炀,陆珂,李浩,林矫矫,傅志强..pVAX1/SjTSP2 DNA疫苗免疫预防小鼠日本血吸虫病的效果评估[J].中国动物传染病学报,2012,20(2):53-58,6.基金项目
公益性行业（农业）科研专项 （）
基本科研业务费（2010JB10）资助 （）
