中国兽医科学2012，Vol.42Issue(1)：35-38,4.
鸭病毒性肝炎病毒RNA聚合酶在真核细胞中的表达及亚细胞定位
Eukaryotic expression and subcellular localization of RNA-dependent RNA polymerase of duck hepatitis A virus
摘要
Abstract
To study the molecular biological characteristics and subcellular localization of RNAdependent RNA polymerase（RdRp） of duck hepatitis A virus（DHAV）,the recombinant plasmid pEGFPRdRp containing RdRp fused with the green fluorescent protein（GFP） was constructed. After transfected into MDCK cells,the expression of RdRp was detected with fluorescence microscope and Western-blotting, and the results showed that RdRp fused with GFP was expressed well in the MDCK cells. Besides, the localization of RdRp in MDCK cells was determined with laser confocal microscope. The results indicated that RdRp was mainly distributed in the nucleus of MDCK cells. And the expressed fusion protein with molecular weight of 80 ku could react with GFP antibody in Western-blotting analysis,demonstrating that the fusion protein possessed immunological activity. The fusion expression vector pEGFP-RdRp was successfully constructed and expressed efficiently in eukaryotic cells,which suggesting that RdRp mainly plays replicase function in nucleus of host cells.关键词
鸭病毒性肝炎病毒/RNA依赖性RNA聚合酶/亚细胞定位Key words
duck hepatitis A virus/RNA-dependent RNA polymerase/subcellular localization分类
农业科技引用本文复制引用
王超,李传峰,陈宗艳,付玉志,昊润,刘光清..鸭病毒性肝炎病毒RNA聚合酶在真核细胞中的表达及亚细胞定位[J].中国兽医科学,2012,42(1):35-38,4.基金项目
公益性农业科研专项(201003012) （201003012）
国家高技术研究发展计划(863)项目(2011AAl0A200) （863）
国家自然科学基金项目(31101848) （31101848）
中央级公益性科研院所基本科研业务费专项(2011JB01) （2011JB01）
