中国兽医科学2012，Vol.42Issue(1)：69-73,5.
伊维菌素对内毒血症小鼠生存率和细胞因子分泌的影响
Effects of ivermectin on survival rate and cytokine secretions in endotoxemic mice
摘要
Abstract
To study the effects of ivermectin on survival rate and serum cytokine secretions in lipopolysaccharide（LPS）-induced endotoxemic mice,C57BL/6 mice were given intraperitoneally with LPS at a lethal dose of 32 mg/kg,and ivermectin of different doses（1,2 and 4 mg/kg） were pre-treated with an orallyadministration 2 h before LPS challenge. Survival of mice was monitored for 7 days twice a day following administration of LPS. The levels of turnout necrosis factor-α（TNF-α）,interleukin-lβ（IL-1β） and interleukin-6（IL-6） in serum of the mice were assessed by sandwich ELISA method,and nitric oxide（NO） levels were measured by nitric acid reduction method at different time following LPS administration. The results showed that ivermectin significantly increased the survival rate in LPS challenged mice, decreased serum levels of TNF-α,IL-1β, IL-6 and NO after LPS challenge. These observations indicated that ivermectin can improve survival rate of endotoxemia mice induced by LPS,which may be mediated by the inhibition of inflammatory cytokine and mediator production.关键词
伊维菌素/脂多糖/生存率/TNF—α/IL—1β/IL-6/一氧化氮Key words
ivermectin/lipopolysaccharide（LPS）/survival rate/TNF-α/IL-1β/IL-6/NO分类
农业科技引用本文复制引用
张雪梅,邓旭明..伊维菌素对内毒血症小鼠生存率和细胞因子分泌的影响[J].中国兽医科学,2012,42(1):69-73,5.基金项目
国家自然科学基金项目(30671586) （30671586）
