中国兽医科学2012，Vol.42Issue(2)：126-131,6.
一株致猪胃溃疡大肠杆菌的分离鉴定
Isolation and identification of enteropathogenic Escherichia coli from swine with gastric ulcer
摘要
Abstract
An infectious disease occurred in a pig farm in Sichuan Province,which was characterized by water-like diarrhea,gastric mucosa shedding and gastric ulcer. To identify the reason,one representative sample was selected for pathogen detection,mesenteric lymph nodes were collected for virus detection,and intestinal contents and liver were collected for the separation of parasites and bacteria. In result,no viruses and parasites were dected but one bacterial strain（SCDC-1） was isolated from the intestinal contents,and confirmed by pathogen purification, mice pathogenic experiment, pig return experiment,and biochemical test. The 16S rDNA sequence of SCDC1 was found to be consistent with that of eight strains of Enterobacteraceae and four strains of Pasteurella deposited in the GenBank. Homology analysis revealed that the identity of 16S rDNA sequence between isolated strain and four E.coli strains were from 99.5% to 99.9%. The pig return experiment proved that the artificially infection can causes the same disease symptoms as before. In addition,sensitive drug screen revealed that the SCDS strain was sensitive to ceftriaxone sodium,cefepime and amikacin. In conclusion,the main pathogen of pig gastric ulcer in this case is Escherichia coli.关键词
猪胃溃疡/大肠杆菌/16S/rDNA/动物回归试验Key words
pig gastric ulcer/Escherichia coli/16S rDNA/animal experiment分类
农业科技引用本文复制引用
代洪波,朱玲,朱长康,史小红,高平,郭万柱,徐志文..一株致猪胃溃疡大肠杆菌的分离鉴定[J].中国兽医科学,2012,42(2):126-131,6.基金项目
教育部“长江学者和创新团队发展计划”创新团队项目 （）
