中国兽医科学2012，Vol.42Issue(2)：138-143,6.
猪源脑心肌炎病毒GXLC株对仔猪的致病性试验
Pathogenic experiment of porcine encephalomyocarditis virus GXLC strain to piglets
摘要
Abstract
To evaluate the viral pathogenicity,the porcine encephalomyocarditis virus（EMCV） GXLC strain was inoculated to 4-week-old healthy piglets. The clinical signs,the pathological changes,the viral loads in heart,brain,spleen and serum,the antibody titers against EMCV in serum were recorded and tested;the mRNA expression levels of IL-1β,TNF-α and IL-6 in heart,brain and spleen were determined.The results showed that the infected piglets had a transient febrile responses,depression and diarrhea without death.At necropsy,excessive pericardiac fluids,enlargement and softness of the heart were observed,and hyperaemia of the brain were also evident.The histopathological observation showed interstitial myocarditis and encephalitis,with significant increase of their inflammatory scores.A large number of viruses were detected in brain,heart,spleen and serum,and the high titers of neutralizing antibody against EMCV were detected in serum.The mRNA expression levels of IL-1β,TNF-α and IL-6 in heart,brain and spleen were significant up regulated,with a closely positive corelationship to time of the obvious clinical manifestations and the macroscopical and histopathological lesions of various organs.The results indicated that porcine EMCV GXLC strain was pathogenic to piglets and the proinflammatory cytokines IL-1β,TNF-α and IL-6 played important roles in the pathogenesis of EMCV.关键词
猪源脑心肌炎病毒/致病性/促炎细胞因子/仔猪Key words
porcine encephalomyocarditis virus/pathogenicity/proinflammatory cytokine/piglet分类
农业科技引用本文复制引用
施开创,胡丽萍,郑敏,粟艳琼,陆文俊,杨荣..猪源脑心肌炎病毒GXLC株对仔猪的致病性试验[J].中国兽医科学,2012,42(2):138-143,6.基金项目
广西科学基金项目 （）
