中国兽医科学2012，Vol.42Issue(2)：144-149,6.
藏獒源犬细小病毒的分离鉴定
Isolation and identification of CPV strains from Tibetan mastiff
摘要
Abstract
To study the epidemiology of canine parvovirus（CPV） in Tibetan mastiff,eight fecal samples were collected by cotton swab from Tibetan mastiff whose CPV test results were positive according to the immune Colloidal Gold kit.Six strains were isolated by cultivation in F81 cells.These strains were identified by HA,TCID50,physicochemical assay.The results showed that the typical cytopathogenic effect（CPE） was spotted in F81 cells from 6 to 13 days after infection.The isolates were able to agglutinate porcine erythrocytes,and the HA titer were from 27 to 211. These strains were all anti-acid（pH3）,anti-heat（60℃） and insensitive to 200mL/L aether and 480mL/L chloroform.The VP2 gene（1755bp） and a 825bp fragment on it were amplified by PCR. Compared the isolates＇ VP2 with 10 reference strains＇（HQ883267.1,FJ222823.1,FJ005214.1,et al） from GenBank,the most similar one was HQ883267.1 with identity of 99.0% to 99.6%.All isolates were proved to be CPV-2a.关键词
犬细小病毒/藏獒/分离/鉴定Key words
CPV/Tibetan mastiff/isolation/identification分类
农业科技引用本文复制引用
梁璐琪,张恒,魏胜男,李思琪,舒龙,钟志军,符华林,曹随忠,胡延春,余树民,李成军,陈英,李万清,彭广能..藏獒源犬细小病毒的分离鉴定[J].中国兽医科学,2012,42(2):144-149,6.基金项目
四川省教育厅重点项目 （）
教育部“长江学者和创新团队发展规划”创新团队项目 （）
四川农业大学双支计划项目 （）
