中国兽医科学2012，Vol.42Issue(2)：166-170,5.
犬瘟热病毒RT-PCR检测方法的建立
Establishment of RT-PCR method for the detection of canine distemper virus
摘要
Abstract
Based on the published sequence of the nucleocapsid gene of Canine distemper virus（CDV） in GenBank,a pair of specific primers was designed for the detection of CDV.As a template with the CDV-ONP strain,and after optimization of the reaction system,a rapid RT-PCR method was established. In result,a fragment of 242bp,as was expected,was amplified from the reverse transcribed template. As low as 20 pg RNA of CDV could be detected accurately and rapidly. This method had good reproducibility and stability and no cross reaction with canine parvovirus,canine coronavirus,rabies virus and Newcastle disease virus. Suspected disease materials had been tested by RT-PCR,and the RT-PCR results were compared with the immune colloidal gold technique with the coincidence rate up to 93.75%. The results showed that this method may be used for clinical diagnosis and laboratory research.关键词
犬瘟热病毒/RT-PCR/检测Key words
canine distemper virus/RT-PCR/detection分类
农业科技引用本文复制引用
郭玲,万莉,马磊,张琦,谢志勇,杨映,舒蕾,陈世界,杨晓农,颜其贵..犬瘟热病毒RT-PCR检测方法的建立[J].中国兽医科学,2012,42(2):166-170,5.基金项目
教育部“长江学者和创新团队发展计划”创新团队项目 （）
成都大熊猫繁育研究基金会项目 （）
四川省科技厅科技支撑计划项目 （）
科技部公益性行业科研专项 （）
